Western blot analysis of lysates from HL-60 cells, using NEPRO Rabbit pAb (STJ11105936) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-NEPRO antibody (263-564 aa) (STJ11105936)

SKU:
STJ11105936
£112.50 - £524.50
Processing The item has been added

Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:100-1:500
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Gene Symbol: Nepro
Gene ID: 212547
Uniprot ID: NEPRO_MOUSE
Immunogen Region: 263-564 aa
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 263-564 of mouse NEPRO (NP_666084.1).
Immunogen Sequence: EQLPKMNEDTLDRLSKPSEQ MTSNPQSTVDLGQPVKACKR TRKEKPLGFDLRAFCTRLGN KATQETNRDFKYSQSKLKTT KLPSQQLRTHWANDTVQRIR KTKTFAQLSEEIEMAIVWSR SKKLKTQATFLGNKLLKSNR FRHVESQGYSLTKKLQCMKT SLCNCLLRGSRTSTSEHPPR QRRSKYKVLSRQRKPQRKLQ STLLKETQQVPEGTLKNTRD SSAKRRCSGTVQRSDVCPN
Function May play a role in cortex development as part of the Notch signaling pathway. Downstream of Notch may repress the expression of proneural genes and inhibit neuronal differentiation thereby maintaining neural progenitors. May also play a role in preimplentation embryo development.
Protein Name Nucleolus And Neural Progenitor Protein
Neural Progenitor Protein
Cellular Localisation Nucleus
Nucleolus
Alternative Antibody Names Anti-Nucleolus And Neural Progenitor Protein antibody
Anti-Neural Progenitor Protein antibody
Anti-Nepro antibody

Information sourced from Uniprot.org