| Host: |
Rabbit |
| Applications: |
WB/ELISA/IHC |
| Reactivity: |
Human/Rat/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Neurofilament medium polypeptide (542-591) is suitable for use in Western Blot, ELISA and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000IHC-p 1:50-300ELISA 2000-20000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
NEFM |
| Gene ID: |
4741 |
| Uniprot ID: |
NFM_HUMAN |
| Immunogen Region: |
542-591 |
| Specificity: |
NF-M Polyclonal Antibody detects endogenous levels of NF-M protein. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from human NF-M. AA range:542-591 |
| Post Translational Modifications | There are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function. Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization. |
| Function | Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks. |
| Protein Name | Neurofilament Medium PolypeptideNf-M160 Kda Neurofilament ProteinNeurofilament 3Neurofilament Triplet M Protein |
| Cellular Localisation | CytoplasmCytoskeletonCell ProjectionAxon |
| Alternative Antibody Names | Anti-Neurofilament Medium Polypeptide antibodyAnti-Nf-M antibodyAnti-160 Kda Neurofilament Protein antibodyAnti-Neurofilament 3 antibodyAnti-Neurofilament Triplet M Protein antibodyAnti-NEFM antibodyAnti-NEF3 antibodyAnti-NFM antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
