Anti-NIN antibody (1-287) (STJ110514)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal NIN (1-287) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NIN |
| Gene ID | 51199 |
| Uniprot ID | NIN_HUMAN |
| Immunogen Region | 1-287 |
| Immunogen Sequence | MDEVEQDQHEARLKELFDSF DTTGTGSLGQEELTDLCHML SLEEVAPVLQQTLLQDNLLG RVHFDQFKEALILILSRTLS NEEHFQEPDCSLEAQPKYVR GGKRYGRRSLPEFQESVEEF PEVTVIEPLDEEARPSHIPA GDCSEHWKTQRSEEYEAEGQ LRFWNPDDLNASQSGSSPPQ DWIEEKLQEVCEDLGITRDG HLNRKKLVSICEQYGLQNVD GEMLEEVFHNLDPDGTMSV |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-287 of human NIN (NP_891991.1). |
Additional Info
| Tissue Specificity | Ubiquitous. Highly expressed in heart and skeletal muscle. Isoform 1 is more expressed than isoform 5. |
| Post Translational Modifications | Phosphorylated by AURKA/Aurora kinase A and PKA kinases but not CK2 or AURKB/ Aurora kinase B. |
| Function | Centrosomal protein required in the positioning and anchorage of the microtubule minus-end in epithelial cells. May also act as a centrosome maturation factor. May play a role in microtubule nucleation, by recruiting the gamma-tubulin ring complex to the centrosome. Overexpression does not perturb nucleation or elongation of microtubules but suppresses release of microtubules. Required for centriole organization and microtubule anchoring at the mother centriole. |
| Protein Name | NineinHnineinGlycogen Synthase Kinase 3 Beta-Interacting ProteinGsk3b-Interacting Protein |
| Database Links | |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeCentrioleComponent Of The Core CentrosomeArranged In A Tubular Conformation With An Open And A Closed End Within The CentrosomeIn The Mother CentrosomeIt Localizes At Both Ends Of The Centrosome TubeIncluding The Site Of Centrosome DuplicationWhile In The Daughter Centrosome It Is Present Only At The Closed EndRequires Pcm1 For Centrosome LocalizationLocalizes To The Subdistal Appendage Region Of The Centriole In A Dctn1-Dependent MannerIsoform 6: CytoplasmSeems To Have A Dominant-Negative Effect On Localization Of Other IsoformsPromoting Their Dissociation From The Centrosome |
| Alternative Antibody Names | Anti-Ninein antibodyAnti-Hninein antibodyAnti-Glycogen Synthase Kinase 3 Beta-Interacting Protein antibodyAnti-Gsk3b-Interacting Protein antibodyAnti-NIN antibodyAnti-KIAA1565 antibody |
Information sourced from Uniprot.org