Anti-NLRC4 antibody (71-170) (STJ11105310)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal NLRC4 (71-170) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | Nlrc4 |
| Gene ID | 268973 |
| Uniprot ID | NLRC4_MOUSE |
| Immunogen Region | 71-170 |
| Immunogen Sequence | KSLENWDYFVYQDLTGQNLS YQVTEEDLNVLAQNLKDLYN SPAFLNFYPLGEDIDIIFNL EKTFTEPIMWKKDHRHHRVE QLTLGSLLEALKSPCLIEGE |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 71-170 of mouse NLRC4. (NP_001028539.1). |
Additional Info
| Tissue Specificity | Expressed by intestinal mononuclear phagocytes. |
| Post Translational Modifications | Phosphorylated at Ser-533 following infection of macrophages with S.typhimurium (Salmonella). Phosphorylation is essential for NLRC4 inflammasome function to promote caspase-1 activation and pyroptosis. PRKCD phosphorylates Ser-533 in vitro. |
| Function | Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis. The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria. It senses pathogenic proteins of the type III secretion system (T3SS) and type IV secretion system (T4SS) such as flagellin and PrgJ-like rod proteins via the Naip proteins (Naip1, Naip2 or Naip5): specific Naip proteins recognize and bind pathogenic proteins, driving assembly and activation of the NLRC4 inflammasome. The NLRC4 inflammasome senses Gram-negative bacteria such as L.pneumophila and P.aeruginosa, enteric pathogens S.typhimurium (Salmonella) and S.flexneri and fungal pathogen C.albicans. In intestine, the NLRC4 inflammasome is able to discriminate between commensal and pathogenic bacteria and specifically drives production of interleukin-1 beta (IL1B) in response to infection by Salmonella or P.aeruginosa. In case of L.pneumophila infection the inflammasome acts by activating caspase-7. |
| Protein Name | Nlr Family Card Domain-Containing Protein 4Caspase Recruitment Domain-Containing Protein 12Ice Protease-Activating FactorIpaf |
| Database Links | |
| Cellular Localisation | CytoplasmCytosolInflammasome |
| Alternative Antibody Names | Anti-Nlr Family Card Domain-Containing Protein 4 antibodyAnti-Caspase Recruitment Domain-Containing Protein 12 antibodyAnti-Ice Protease-Activating Factor antibodyAnti-Ipaf antibodyAnti-Nlrc4 antibodyAnti-Card12 antibodyAnti-Ipaf antibody |
Information sourced from Uniprot.org