| Host: | Rabbit |
| Applications: | WB/IF/ICC/IP/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | SRM |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:1000-1:5000 IF/ICC:1:50-1:200 IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirement |
| Gene Symbol: | NNMT |
| Gene ID: | 4837 |
| Uniprot ID: | NNMT_HUMAN |
| Immunogen Region: | 1-264 aa |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-264 of human NNMT (NP_006160.1). |
| Immunogen Sequence: | MESGFTSKDTYLSHFNPRDY LEKYYKFGSRHSAESQILKH LLKNLFKIFCLDGVKGDLLI DIGSGPTIYQLLSACESFKE IVVTDYSDQNLQELEKWLKK EPEAFDWSPVVTYVCDLEGN RVKGPEKEEKLRQAVKQVLK CDVTQSQPLGAVPLPPADCV LSTLCLDAACPDLPTYCRAL RNLGSLLKPGGFLVIMDALK SSYYMIGEQKFSSLPLGREA VEAAVKEAGYTIEWFEVIS |
| Tissue Specificity | Predominantly expressed in the liver. A lower expression is seen in the kidney, lung, skeletal muscle, placenta and heart. Not detected in the brain or pancreas. |
| Post Translational Modifications | Deiminated by PADI1 and PADI2. |
| Function | Catalyzes the N-methylation of nicotinamide using the universal methyl donor S-adenosyl-L-methionine to form N1-methylnicotinamide and S-adenosyl-L-homocysteine, a predominant nicotinamide/vitamin B3 clearance pathway. Plays a central role in regulating cellular methylation potential, by consuming S-adenosyl-L-methionine and limiting its availability for other methyltransferases. Actively mediates genome-wide epigenetic and transcriptional changes through hypomethylation of repressive chromatin marks, such as H3K27me3. In a developmental context, contributes to low levels of the repressive histone marks that characterize pluripotent embryonic stem cell pre-implantation state. Acts as a metabolic regulator primarily on white adipose tissue energy expenditure as well as hepatic gluconeogenesis and cholesterol biosynthesis. In white adipocytes, regulates polyamine flux by consuming S-adenosyl-L-methionine which provides for propylamine group in polyamine biosynthesis, whereas by consuming nicotinamide controls NAD(+) levels through the salvage pathway. Via its product N1-methylnicotinamide regulates protein acetylation in hepatocytes, by repressing the ubiquitination and increasing the stability of SIRT1 deacetylase. Can also N-methylate other pyridines structurally related to nicotinamide and play a role in xenobiotic detoxification. |
| Protein Name | Nicotinamide N-Methyltransferase |
| Database Links | Reactome: R-HSA-156581 Reactome: R-HSA-197264 Reactome: R-HSA-2408508 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Nicotinamide N-Methyltransferase antibody Anti-NNMT antibody |
Information sourced from Uniprot.org