Anti-NOTCH3 antibody (1520-1640) (STJ24789)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal NOTCH3 (1520-1640) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:200-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NOTCH3 |
| Gene ID | 4854 |
| Uniprot ID | NOTC3_HUMAN |
| Immunogen Region | 1520-1640 |
| Immunogen Sequence | PPEELLRSSADFLQRLSAIL RTSLRFRLDAHGQAMVFPYH RPSPGSEPRARRELAPEVIG SVVMLEIDNRLCLQSPENDH CFPDAQSAADYLGALSAVER LDFPYPLRDVRGEPLEPPEP S |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1520-1640 of human NOTCH3 (NP_000426.2). |
Additional Info
| Tissue Specificity | Ubiquitously expressed in fetal and adult tissues. |
| Post Translational Modifications | Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane. Phosphorylated. Hydroxylated by HIF1AN. |
| Function | Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. |
| Protein Name | Neurogenic Locus Notch Homolog Protein 3Notch 3 Cleaved Into - Notch 3 Extracellular Truncation - Notch 3 Intracellular Domain |
| Database Links | Reactome: R-HSA-1912399Reactome: R-HSA-1912408Reactome: R-HSA-1912420Reactome: R-HSA-350054Reactome: R-HSA-5083630Reactome: R-HSA-9013507Reactome: R-HSA-9013508Reactome: R-HSA-9017802 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinNotch 3 Intracellular Domain: NucleusFollowing Proteolytical Processing Nicd Is Translocated To The Nucleus |
| Alternative Antibody Names | Anti-Neurogenic Locus Notch Homolog Protein 3 antibodyAnti-Notch 3 Cleaved Into - Notch 3 Extracellular Truncation - Notch 3 Intracellular Domain antibodyAnti-NOTCH3 antibody |
Information sourced from Uniprot.org