Anti-NOTCH4 Antibody

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Product name Anti-NOTCH4 Antibody
Short Description Rabbit polyclonal against NOTCH4
Description This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor may play a role in vascular, renal and hepatic development. Mutations in this gene may be associated with schizophrenia. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.
Applications WB
Dilution range WB 1:500 - 1:2000
Protein Name Neurogenic locus notch homolog protein 4
Notch 4
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1824-2003 of human NOTCH4 (NP_004548.3).
Storage Instruction Store at -20C. Avoid freeze / thaw cycles.
Note For Research Use Only (RUO).
Validated Application WB
Host Rabbit
Clonality Polyclonal
Reactivity Human, Mouse, Rat
Conjugation Unconjugated
Purification Affinity purification
Isotype IgG
Formulation PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Gene ID 4855
Gene Symbol NOTCH4
Molecular Weight 209.622 kDa
Database Links HGNC:7884
Alternative Names Neurogenic locus notch homolog protein 4
Notch 4
Function Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination, Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus, Affects the implementation of differentiation, proliferation and apoptotic programs, May regulate branching morphogenesis in the developing vascular system ,
Post-translational Modifications Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form, Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC), Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT), This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane ,
Cellular Localization Cell membrane
Tissue Specificity Highly expressed in the heart, moderately in the lung and placenta and at low levels in the liver, skeletal muscle, kidney, pancreas, spleen, lymph node, thymus, bone marrow and fetal liver, No expression was seen in adult brain or peripheral blood leukocytes
Swiss-Prot Key Q99466
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Ting-Yuan Liu, Yu-Chia Chen, Yuh-Jyh Jong, Huai-Jen Tsai, Chien-Chin Lee, Ya-Sian Chang, Jan-Gowth Chang, Yung-Fu Chang. "Muscle developmental defects in heterogeneous nuclear Ribonucleoprotein A1 knockout mice". Open Biol. 2017 7 160303;  Published 11 January 2017  doi:10.1098/rsob.160303
R. Ferreira,*a   M. C. Fonseca,a   T. Santos,a   J. Sargento-Freitas,bc   R. Tjeng,d   F. Paiva,d   M. Castelo-Branco,ae   L. S. Ferreiracf and   L. Bernardino*. Retinoic acid-loaded polymeric nanoparticles enhance vascular regulation of neural stem cell survival and differentiation after ischaemia. Nanoscale. Issue 15, 2016.   doi:10.1039/C5NR09077F

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