• Western blot analysis of various lysates using NSUN3 Rabbit polyclonal antibody (STJ114758) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-NSUN3 antibody (1-120) (STJ114758)

SKU:
STJ114758

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NSUN3 (1-120) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NSUN3
Gene ID: 63899
Uniprot ID: NSUN3_HUMAN
Immunogen Region: 1-120
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human NSUN3 (NP_071355.1).
Immunogen Sequence: MLTQLKAKSEGKLAKQICKV VLDHFEKQYSKELGDAWNTV REILTSPSCWQYAVLLNRFN YPFELEKDLHLKGYHTLSQG SLPNYPKSVKCYLSRTPGRI PSERHQIGNLKKYYLLNAAS
Function Mitochondrial tRNA methyltransferase that mediates methylation of cytosine to 5-methylcytosine (m5C) at position 34 of mt-tRNA(Met). mt-tRNA(Met) methylation at cytosine(34) takes place at the wobble position of the anticodon and initiates the formation of 5-formylcytosine (f(5)c) at this position. mt-tRNA(Met) containing the f(5)c modification at the wobble position enables recognition of the AUA codon in addition to the AUG codon, expanding codon recognition in mitochondrial translation.
Protein Name Trna
Cytosine(34-C(5-Methyltransferase - Mitochondrial
Nol1/Nop2/Sun Domain Family Member 3
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Trna antibody
Anti-Cytosine(34-C(5-Methyltransferase - Mitochondrial antibody
Anti-Nol1/Nop2/Sun Domain Family Member 3 antibody
Anti-NSUN3 antibody
Anti-MSTP077 antibody
Anti-UG0651E06 antibody

Information sourced from Uniprot.org

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