| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ODF2 |
| Gene ID: | 4957 |
| Uniprot ID: | ODFP2_HUMAN |
| Immunogen Region: | 630-829 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 630-829 of human ODF2 (NP_702911.1). |
| Immunogen Sequence: | IISDLRSRIEHQGDKLEMAR EKHQASQKENKQLSLKVDEL ERKLEATSAQNIEFLQVIAK REEAIHQSQLRLEEKTRECG TLARQLESAIEDARRQVEQT KEHALSKERAAQNKILDLET QLSRTKTELSQLRRSRDDAD RRYQSRLQDLKDRLEQSEST NRSMQNYVQFLKSSYANVFG DGPYSTFLTSSPIRSRSPPA |
| Tissue Specificity | Testis-specific (at protein level). Highly expressed in cytoplasm of step 2 round spermatids. Detected in the middle piece and extends to about half the principal piece of the sperm tails. |
| Post Translational Modifications | Tyrosine phosphorylated. Phosphorylated by TSSK4 on Ser-95. |
| Function | Seems to be a major component of sperm tail outer dense fibers (ODF). ODFs are filamentous structures located on the outside of the axoneme in the midpiece and principal piece of the mammalian sperm tail and may help to maintain the passive elastic structures and elastic recoil of the sperm tail. May have a modulating influence on sperm motility. Functions as a general scaffold protein that is specifically localized at the distal/subdistal appendages of mother centrioles. Component of the centrosome matrix required for the localization of PLK1 and NIN to the centrosomes. Required for the formation and/or maintenance of normal CETN1 assembly. |
| Protein Name | Outer Dense Fiber Protein 2 Cenexin Outer Dense Fiber Of Sperm Tails Protein 2 |
| Database Links | Reactome: R-HSA-2565942 Reactome: R-HSA-380259 Reactome: R-HSA-380270 Reactome: R-HSA-380284 Reactome: R-HSA-380320 Reactome: R-HSA-5620912 Reactome: R-HSA-8854518 |
| Cellular Localisation | Cytoplasm Cytoskeleton Microtubule Organizing Center Centrosome Cell Projection Cilium Centriole Spindle Pole Flagellum Localized At The Microtubule Organizing Centers In Interphase And Spindle Poles In Mitosis Localized At The Distal/Subdistal Appendages Of Mother Centrioles |
| Alternative Antibody Names | Anti-Outer Dense Fiber Protein 2 antibody Anti-Cenexin antibody Anti-Outer Dense Fiber Of Sperm Tails Protein 2 antibody Anti-ODF2 antibody |
Information sourced from Uniprot.org