• Western blot analysis of extracts of various cell lines, using ONECUT1 antibody (STJ114647) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ONECUT1 antibody (167-292) (STJ114647)

SKU:
STJ114647

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ONECUT1 (167-292) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ONECUT1
Gene ID: 3175
Uniprot ID: HNF6_HUMAN
Immunogen Region: 167-292
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 167-292 of human ONECUT1 (NP_004489.1).
Immunogen Sequence: PYHKDVAGMGQSLSPLSSSG LGSIHNSQQGLPHYAHPGAA MPTDKMLTPNGFEAHHPAML GRHGEQHLTPTSAGMVPING LPPHHPHAHLNAQGHGQLLG TAREPNPSVTGAQVSNGSNS GQMEEI
Tissue Specificity Highly expressed in liver.lower expression in testis and skin.
Function Transcriptional activator. Binds the consensus sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR. Important for liver genes transcription.
Protein Name Hepatocyte Nuclear Factor 6
Hnf-6
One Cut Domain Family Member 1
One Cut Homeobox 1
Database Links Reactome: R-HSA-210744
Reactome: R-HSA-210747
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Hepatocyte Nuclear Factor 6 antibody
Anti-Hnf-6 antibody
Anti-One Cut Domain Family Member 1 antibody
Anti-One Cut Homeobox 1 antibody
Anti-ONECUT1 antibody
Anti-HNF6 antibody
Anti-HNF6A antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance