• Western blot analysis of lysates from ovary cancer, using MEK2 (Phospho-Thr394) Antibody. The lane on the right is blocked with the phospho peptide.
  • Western blot analysis of VEC cells using Phospho-MEK-2 (T394) Polyclonal Antibody diluted at 1：2000
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MEK2 (Phospho-Thr394) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of various cells using Phospho-MEK-2 (T394) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-MAP2K2-Thr394 antibody (261-310 aa) (STJ90329)

SKU:
STJ90329

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/IP/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Dual specificity mitogen-activated protein kinase kinase 2-Thr394 (261-310 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IP 2-5 ug mg/lysate
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAP2K2
Gene ID: 5605
Uniprot ID: MP2K2_HUMAN
Immunogen Region: 261-310 aa
Specificity: Phospho-MEK-2 (T394) Polyclonal Antibody detects endogenous levels of MEK-2 protein only when phosphorylated at T394.
Immunogen: The antiserum was produced against synthesized peptide derived from the human MEK2 around the phosphorylation site of Thr394 at the amino acid range 261-310
Function Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases. Activates BRAF in a KSR1 or KSR2-dependent manner.by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation.
Protein Name Dual Specificity Mitogen-Activated Protein Kinase Kinase 2
Map Kinase Kinase 2
Mapkk 2
Erk Activator Kinase 2
Mapk/Erk Kinase 2
Mek 2
Database Links Reactome: R-HSA-112411
Reactome: R-HSA-170968
Reactome: R-HSA-445144
Reactome: R-HSA-5210891
Reactome: R-HSA-5673000
Reactome: R-HSA-5674135
Reactome: R-HSA-5674499
Reactome: R-HSA-6802946
Reactome: R-HSA-6802948
Reactome: R-HSA-6802952
Reactome: R-HSA-6802955
Reactome: R-HSA-9649948
Reactome: R-HSA-9652169
Reactome: R-HSA-9656223
Cellular Localisation Cytoplasm
Membrane
Peripheral Membrane Protein
Membrane Localization Is Probably Regulated By Its Interaction With Ksr1
Alternative Antibody Names Anti-Dual Specificity Mitogen-Activated Protein Kinase Kinase 2 antibody
Anti-Map Kinase Kinase 2 antibody
Anti-Mapkk 2 antibody
Anti-Erk Activator Kinase 2 antibody
Anti-Mapk/Erk Kinase 2 antibody
Anti-Mek 2 antibody
Anti-MAP2K2 antibody
Anti-MEK2 antibody
Anti-MKK2 antibody
Anti-PRKMK2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance