| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse/Rat/Monkey/Pig |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Phospho-Phosphatidylinositol 3-kinase regulatory subunit alpha and Phosphatidylinositol 3-kinase regulatory subunit gamma-Tyr467/199 (436-485 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluores |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:10000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PIK3R3PIK3R1 |
| Gene ID: | 85035295 |
| Uniprot ID: | P55G_HUMANP85A_HUMAN |
| Immunogen Region: | 436-485 aa |
| Specificity: | Phospho-PI 3-kinase p85/p55 (Y467/199) Polyclonal Antibody detects endogenous levels of PI 3-kinase p85/p55 protein only when phosphorylated at Y467/199. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human PI3-kinase p85-alpha/gamma around the phosphorylation site of Tyr467/199 at the amino acid range 436-485 |
| Post Translational Modifications | Polyubiquitinated in T-cells by CBLB.which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation. Phosphorylated. Tyrosine phosphorylated in response to signaling by FGFR1, FGFR2, FGFR3 and FGFR4. Phosphorylated by CSF1R. Phosphorylated by ERBB4. Phosphorylated on tyrosine residues by TEK/TIE2. Dephosphorylated by PTPRJ. Phosphorylated by PIK3CA at Ser-608.phosphorylation is stimulated by insulin and PDGF. The relevance of phosphorylation by PIK3CA is however unclear. Phosphorylated in response to KIT and KITLG/SCF. Phosphorylated by FGR. |
| Function | Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling. Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement. |
| Protein Name | Phosphatidylinositol 3-Kinase Regulatory Subunit AlphaPi3-Kinase Regulatory Subunit AlphaPi3k Regulatory Subunit AlphaPtdins-3-Kinase Regulatory Subunit AlphaPhosphatidylinositol 3-Kinase 85 Kda Regulatory Subunit AlphaPi3-Kinase Subunit P85-AlphaPtdins-3-Kinase Regulatory Subunit P85-Alpha |
| Database Links | Reactome: R-HSA-109704Reactome: R-HSA-112399Reactome: R-HSA-114604Reactome: R-HSA-1236382Reactome: R-HSA-1250342Reactome: R-HSA-1257604Reactome: R-HSA-1266695Reactome: R-HSA-1433557Reactome: R-HSA-1660499Reactome: R-HSA-180292Reactome: R-HSA-1839117Reactome: R-HSA-186763Reactome: R-HSA-1963642Reactome: R-HSA-198203Reactome: R-HSA-202424Reactome: R-HSA-2029485Reactome: R-HSA-210993Reactome: R-HSA-2219530Reactome: R-HSA-2424491Reactome: R-HSA-2730905Reactome: R-HSA-373753Reactome: R-HSA-388841Reactome: R-HSA-389357Reactome: R-HSA-416476Reactome: R-HSA-430116Reactome: R-HSA-4420097Reactome: R-HSA-512988Reactome: R-HSA-5637810Reactome: R-HSA-5654689Reactome: R-HSA-5654695Reactome: R-HSA-5654710Reactome: R-HSA-5654720Reactome: R-HSA-5655253Reactome: R-HSA-5655291Reactome: R-HSA-5655302Reactome: R-HSA-5673001Reactome: R-HSA-6785807Reactome: R-HSA-6811558Reactome: R-HSA-8851907Reactome: R-HSA-8853334Reactome: R-HSA-8853338Reactome: R-HSA-8853659Reactome: R-HSA-9009391Reactome: R-HSA-9027276Reactome: R-HSA-9028335Reactome: R-HSA-912526Reactome: R-HSA-912631Reactome: R-HSA-9603381Reactome: R-HSA-9607240Reactome: R-HSA-9664565Reactome: R-HSA-9665348Reactome: R-HSA-9670439Reactome: R-HSA-9673767Reactome: R-HSA-9673770Reactome: R-HSA-983695 |
| Alternative Antibody Names | Anti-Phosphatidylinositol 3-Kinase Regulatory Subunit Alpha antibodyAnti-Pi3-Kinase Regulatory Subunit Alpha antibodyAnti-Pi3k Regulatory Subunit Alpha antibodyAnti-Ptdins-3-Kinase Regulatory Subunit Alpha antibodyAnti-Phosphatidylinositol 3-Kinase 85 Kda Regulatory Subunit Alpha antibodyAnti-Pi3-Kinase Subunit P85-Alpha antibodyAnti-Ptdins-3-Kinase Regulatory Subunit P85-Alpha antibodyAnti-PIK3R1 antibodyAnti-GRB1 antibody |
Information sourced from Uniprot.org
