| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-POLR3A (1-200) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
POLR3A |
| Gene ID: |
11128 |
| Uniprot ID: |
RPC1_HUMAN |
| Immunogen Region: |
1-200 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human POLR3A (NP_008986.2). |
| Immunogen Sequence: |
MVKEQFRETDVAKKISHICF GMKSPEEMRQQAHIQVVSKN LYSQDNQHAPLLYGVLDHRM GTSEKDRPCETCGKNLADCL GHYGYIDLELPCFHVGYFRA VIGILQMICKTCCHIMLSQE EKKQFLDYLKRPGLTYLQKR GLKKKISDKCRKKNICHHCG AFNGTVKKCGLLKIIHEKYK TNKKVVDPIVSNFLQSFETA |
| Tissue Specificity | Expressed in the brain, in the cortex and the white matter (at protein level). |
| Function | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. |
| Protein Name | Dna-Directed Rna Polymerase Iii Subunit Rpc1Rna Polymerase Iii Subunit C1Dna-Directed Rna Polymerase Iii Largest SubunitDna-Directed Rna Polymerase Iii Subunit ARna Polymerase Iii 155 Kda SubunitRpc155Rna Polymerase Iii Subunit C160 |
| Database Links | Reactome: R-HSA-1834949Reactome: R-HSA-73780Reactome: R-HSA-73980Reactome: R-HSA-749476Reactome: R-HSA-76061Reactome: R-HSA-76066Reactome: R-HSA-76071 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Dna-Directed Rna Polymerase Iii Subunit Rpc1 antibodyAnti-Rna Polymerase Iii Subunit C1 antibodyAnti-Dna-Directed Rna Polymerase Iii Largest Subunit antibodyAnti-Dna-Directed Rna Polymerase Iii Subunit A antibodyAnti-Rna Polymerase Iii 155 Kda Subunit antibodyAnti-Rpc155 antibodyAnti-Rna Polymerase Iii Subunit C160 antibodyAnti-POLR3A antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
