Anti-PRKAB1 antibody [1A7-E11-E9] (STJ99185)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG2a
ImmunogenPurified recombinant human AMPK beta 1 protein fragments expressed in E.coli.
General Information
| Short Description | Mouse monoclonal anti-5 AMP-activated protein kinase subunit beta-1 for use in WB, ICC, IP and IHC in Human, Mouse, Rat and Monkey samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ICC/IP/IHC |
| Host | Mouse |
| Reactivity | Human/Mouse/Rat/Monkey |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 1A7-E11-E9 |
| Isotype | IgG2a |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:1000ICC 1:100 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PRKAB1 |
| Gene ID | 5564 |
| Uniprot ID | AAKB1_HUMAN |
| Immunogen | Purified recombinant human AMPK beta 1 protein fragments expressed in E.coli. |
| Specificity | This antibody detects endogenous levels of AMPK beta 1 and does not cross-react with related proteins. |
Additional Info
| Function | Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton.probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3). |
| Protein Name | 5'-Amp-Activated Protein Kinase Subunit Beta-1Ampk Subunit Beta-1Ampkb |
| Database Links | Reactome: R-HSA-1445148Reactome: R-HSA-1632852Reactome: R-HSA-2151209Reactome: R-HSA-380972Reactome: R-HSA-5628897Reactome: R-HSA-6804756Reactome: R-HSA-9613354Reactome: R-HSA-9619483 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-5'-Amp-Activated Protein Kinase Subunit Beta-1 antibodyAnti-Ampk Subunit Beta-1 antibodyAnti-Ampkb antibodyAnti-PRKAB1 antibodyAnti-AMPK antibody |
Information sourced from Uniprot.org