Anti-RHOC antibody (94-193) (STJ11104547)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Rho-Related Gtp-Binding Protein Rhoc (94-193) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RHOC |
| Gene ID | 389 |
| Uniprot ID | RHOC_HUMAN |
| Immunogen Region | 94-193 |
| Immunogen Sequence | NIPEKWTPEVKHFCPNVPII LVGNKKDLRQDEHTRRELAK MKQEPVRSEEGRDMANRISA FGYLECSAKTKEGVREVFEM ATRAGLQVRKNKRRRGCPIL |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 94-193 of human RhoC (NP_786886.1). |
Additional Info
| Post Translational Modifications | (Microbial infection) Glycosylated at Tyr-34 by Photorhabdus asymbiotica toxin PAU_02230. Mono-O-GlcNAcylation by PAU_02230 inhibits downstream signaling by an impaired interaction with diverse regulator and effector proteins of Rho and leads to actin disassembly. (Microbial infection) Glucosylated at Thr-37 by C.difficile toxins TcdA and TcdB in the colonic epithelium. Monoglucosylation completely prevents the recognition of the downstream effector, blocking the GTPases in their inactive form, leading to actin cytoskeleton disruption. |
| Function | Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Serves as a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Regulates apical junction formation in bronchial epithelial cells. |
| Protein Name | Rho-Related Gtp-Binding Protein RhocRho Cdna Clone 9H9 |
| Database Links | Reactome: R-HSA-416482Reactome: R-HSA-416572Reactome: R-HSA-5625740Reactome: R-HSA-5625900Reactome: R-HSA-5627117Reactome: R-HSA-5663220Reactome: R-HSA-5666185Reactome: R-HSA-9013106 |
| Cellular Localisation | Cell MembraneLipid-AnchorCytoplasmic SideCleavage FurrowTranslocates To The Equatorial Region Before Furrow Formation In A Ect2-Dependent Manner |
| Alternative Antibody Names | Anti-Rho-Related Gtp-Binding Protein Rhoc antibodyAnti-Rho Cdna Clone 9 antibodyAnti-H9 antibodyAnti-RHOC antibodyAnti-ARH9 antibodyAnti-ARHC antibody |
Information sourced from Uniprot.org