| Applications: | WB/IHC-P/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:1000-1:5000IHC-P:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RPL22 |
| Gene ID: | 6146 |
| Uniprot ID: | RL22_HUMAN |
| Immunogen Region: | 1-122 |
| Immunogen Sequence: | MAPVKKLVVKGGKKKKQVLK FTLDCTHPVEDGIMDAANFE QFLQERIKVNGKAGNLGGGV VTIERSKSKITVTSEVPFSK RYLKYLTKKYLKKNNLRDWL RVVANSKESYELRYFQINQD EE |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-122 of human RPL22 (NP_000974.1). |
Information sourced from Uniprot.org
![Western blot analysis of various lysates using [KO Validated] RPL22 Rabbit pAb (STJ113622) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/92984/409490/STJ113622_1__66397.1744895912.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] RPL22 Rabbit pAb (STJ113622) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.")
![Western blot analysis of lysates from wild type (WT) and RPL22 knockout (KO) 293T cells, using [KO Validated] RPL22 Rabbit pAb (STJ113622) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/92984/409491/STJ113622_2__20351.1744895913.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and RPL22 knockout (KO) 293T cells, using [KO Validated] RPL22 Rabbit pAb (STJ113622) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.")
![Immunohistochemistry analysis of paraffin-embedded Rat lung using [KO Validated] RPL22 Rabbit pAb (STJ113622) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0. 01M PBS Buffer (pH 7. 2) prior to immunohistochemistry staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/92984/409492/STJ113622_3__93315.1744895914.jpg?c=1 "Immunohistochemistry analysis of paraffin-embedded Rat lung using [KO Validated] RPL22 Rabbit pAb (STJ113622) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0. 01M PBS Buffer (pH 7. 2) prior to immunohistochemistry staining.")
![Western blot analysis of various lysates using [KO Validated] RPL22 Rabbit pAb (STJ113622) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/92984/409490/STJ113622_1__66397.1744895912.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] RPL22 Rabbit pAb (STJ113622) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.")
![Western blot analysis of lysates from wild type (WT) and RPL22 knockout (KO) 293T cells, using [KO Validated] RPL22 Rabbit polyclonal antibody (STJ11100742) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90S.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89680/358173/STJ11100742_1__83977.1713122480.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and RPL22 knockout (KO) 293T cells, using [KO Validated] RPL22 Rabbit polyclonal antibody (STJ11100742) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90S.")
