| Host: | Rabbit |
| Applications: | WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Sharpin is suitable for use in Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | SHARPIN |
| Gene ID: | 81858 |
| Uniprot ID: | SHRPN_HUMAN |
| Specificity: | This antibody detects endogenous levels of SHARPIN at Human, Mouse, Rat |
| Immunogen: | Synthesized peptide derived from human SHARPIN |
| Function | Component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. The LUBAC complex is also involved in innate immunity by conjugating linear polyubiquitin chains at the surface of bacteria invading the cytosol to form the ubiquitin coat surrounding bacteria. LUBAC is not able to initiate formation of the bacterial ubiquitin coat, and can only promote formation of linear polyubiquitins on pre-existing ubiquitin. The bacterial ubiquitin coat acts as an 'eat-me' signal for xenophagy and promotes NF-kappa-B activation. Together with OTULIN, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis. |
| Protein Name | Sharpin Shank-Associated Rh Domain-Interacting Protein Shank-Interacting Protein-Like 1 Hsipl1 |
| Database Links | Reactome: R-HSA-5357786 Reactome: R-HSA-5357905 Reactome: R-HSA-5357956 Reactome: R-HSA-6794361 |
| Cellular Localisation | Cytoplasm Cytosol Synapse Enriched At Synaptic Sites In Mature Neurons Where It Colocalizes With Shank1 |
| Alternative Antibody Names | Anti-Sharpin antibody Anti-Shank-Associated Rh Domain-Interacting Protein antibody Anti-Shank-Interacting Protein-Like 1 antibody Anti-Hsipl1 antibody Anti-SHARPIN antibody Anti-SIPL1 antibody Anti-PSEC0216 antibody |
Information sourced from Uniprot.org