• Western blot analysis of extracts of Raji cells, using SPIB antibody (STJ29587) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-SPIB antibody (1-262) (STJ29587)

SKU:
STJ29587

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SPIB (1-262) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SPIB
Gene ID: 6689
Uniprot ID: SPIB_HUMAN
Immunogen Region: 1-262
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-262 of human SPIB (NP_003112.2).
Immunogen Sequence: MLALEAAQLDGPHFSCLYPD GVFYDLDSCKHSSYPDSEGA PDSLWDWTVAPPVPATPYEA FDPAAAAFSHPQAAQLCYEP PTYSPAGNLELAPSLEAPGP GLPAYPTENFASQTLVPPAY APYPSPVLSEEEDLPLDSPA LEVSDSESDEALVAGPEGKG SEAGTRKKLRLYQFLLGLLT RGDMRECVWWVEPGAGVFQF SSKHKELLARRWGQQKGNRK RMTYQKLARALRNYAKTGE
Tissue Specificity Expressed in plasmacytoid dendritic cells (pDCs) and B-cells, not expressed in T-cells or granulocytes. May also be enriched in stem cell populations of the liver.
Function Sequence specific transcriptional activator which binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. Promotes development of plasmacytoid dendritic cells (pDCs), also known as type 2 DC precursors (pre-DC2) or natural interferon (IFN)-producing cells. These cells have the capacity to produce large amounts of interferon and block viral replication. May be required for B-cell receptor (BCR) signaling, which is necessary for normal B-cell development and antigenic stimulation.
Protein Name Transcription Factor Spi-B
Cellular Localisation Isoform 1: Nucleus
Isoform 2: Cytoplasm
Alternative Antibody Names Anti-Transcription Factor Spi-B antibody
Anti-SPIB antibody

Information sourced from Uniprot.org

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