Anti-Sprouty 2 antibody (216-315) [S5MR] (STJ11103625)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal Sprouty 2 (216-315) antibody for use in WB, IHC-P and ELISA in human and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S5MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SPRY2 |
| Gene ID | 10253 |
| Uniprot ID | SPY2_HUMAN |
| Immunogen Region | 216-315 |
| Immunogen Sequence | GTCVCCVKGLFYHCSNDDED NCADNPCSCSQSHCCTRWSA MGVMSLFLPCLWCYLPAKGC LKLCQGCYDRVNRPGCRCKN SNTVCCKVPTVPPRNFEKPT |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 216-315 of human Sprouty 2 (O43597). |
Additional Info
| Post Translational Modifications | Cleaved at Pro-144 by the prolyl endopeptidase FAP (seprase) activity (in vitro). |
| Function | Antagonist of fibroblast growth factor (FGF) pathways via inhibition of FGF-mediated phosphorylation of ERK1/2. Thereby acts as an antagonist of FGF-induced retinal lens fiber differentiation, may inhibit limb bud outgrowth and may negatively modulate respiratory organogenesis. Inhibits TGFB-induced epithelial-to-mesenchymal transition in retinal lens epithelial cells. Inhibits CBL/C-CBL-mediated EGFR ubiquitination. |
| Protein Name | Protein Sprouty Homolog 2Spry-2 |
| Database Links | Reactome: R-HSA-1295596Reactome: R-HSA-182971 |
| Cellular Localisation | CytoplasmCytoskeletonCell ProjectionRuffle MembraneAssociated With Microtubules In Unstimulated Cells But Is Translocated To The Membrane Ruffles In Cells Stimulated With Egf (Epidermal Growth Factor) |
| Alternative Antibody Names | Anti-Protein Sprouty Homolog 2 antibodyAnti-Spry-2 antibodyAnti-SPRY2 antibody |
Information sourced from Uniprot.org