![Western blot analysis of lysates from wild type (WT) and SUCLG2 knockout (KO) 293T (KO) cells, using [KO Validated] SUCLG2 Rabbit pAb (STJ116335) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/95318/410028/STJ116335_1__72895.1744896481.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and SUCLG2 knockout (KO) 293T (KO) cells, using [KO Validated] SUCLG2 Rabbit pAb (STJ116335) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.")
![Immunohistochemistry analysis of paraffin-embedded Human liver cancer using [KO Validated] SUCLG2 Rabbit pAb (STJ116335) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0. 01M PBS Buffer (pH 7. 2) prior to immunohistochemistry staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/95318/410029/STJ116335_2__92098.1744896482.jpg?c=1 "Immunohistochemistry analysis of paraffin-embedded Human liver cancer using [KO Validated] SUCLG2 Rabbit pAb (STJ116335) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0. 01M PBS Buffer (pH 7. 2) prior to immunohistochemistry staining.")
![Immunohistochemistry analysis of paraffin-embedded Human lung cancer using [KO Validated] SUCLG2 Rabbit pAb (STJ116335) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0. 01M PBS Buffer (pH 7. 2) prior to immunohistochemistry staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/95318/410030/STJ116335_3__56183.1744896482.jpg?c=1 "Immunohistochemistry analysis of paraffin-embedded Human lung cancer using [KO Validated] SUCLG2 Rabbit pAb (STJ116335) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0. 01M PBS Buffer (pH 7. 2) prior to immunohistochemistry staining.")
![Immunofluorescence analysis of C6 cells using [KO Validated] SUCLG2 Rabbit pAb (STJ116335) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/95318/410031/STJ116335_4__47807.1744896483.jpg?c=1 "Immunofluorescence analysis of C6 cells using [KO Validated] SUCLG2 Rabbit pAb (STJ116335) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunofluorescence analysis of U-2 OS cells using [KO Validated] SUCLG2 Rabbit pAb (STJ116335) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/95318/410032/STJ116335_5__95651.1744896483.jpg?c=1 "Immunofluorescence analysis of U-2 OS cells using [KO Validated] SUCLG2 Rabbit pAb (STJ116335) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western blot analysis of lysates from wild type (WT) and SUCLG2 knockout (KO) 293T (KO) cells, using [KO Validated] SUCLG2 Rabbit pAb (STJ116335) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/95318/410028/STJ116335_1__72895.1744896481.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and SUCLG2 knockout (KO) 293T (KO) cells, using [KO Validated] SUCLG2 Rabbit pAb (STJ116335) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.")
Anti-SUCLG2 antibody (50-300) (STJ116335)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal SUCLG2 (50-300) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:5000IHC-P:1:50-1:200IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SUCLG2 |
| Gene ID | 8801 |
| Uniprot ID | SUCB2_HUMAN |
| Immunogen Region | 50-300 |
| Immunogen Sequence | SDNGVRVQRFFVADTANEAL EAAKRLNAKEIVLKAQILAG GRGKGVFNSGLKGGVHLTKD PNVVGQLAKQMIGYNLATKQ TPKEGVKVNKVMVAEALDIS RETYLAILMDRSCNGPVLVG SPQGGVDIEEVAASNPELIF KEQIDIFEGIKDSQAQRMAE NLGFVGPLKSQAADQITKLY NLFLKIDATQVEVNPFGETP EGQVVCFDAKINFDDNAEFR QKDIFAMDDKSENEPIENE |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 50-300 of human SUCLG2 (NP_003839.2). |
Additional Info
| Tissue Specificity | Mainly expressed in liver, kidney, heart, spleen and skeletal muscle. Also found in intestine and colon, and in low amounts in lung, brain, prostate, testis and ovary. |
| Function | GTP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit. |
| Protein Name | Succinate--Coa Ligase Gdp-Forming Subunit Beta - MitochondrialGtp-Specific Succinyl-Coa Synthetase Subunit BetaG-ScsGtpscsSuccinyl-Coa Synthetase Beta-G ChainScs-Betag |
| Database Links | Reactome: R-HSA-71403Reactome: R-HSA-9837999 |
| Cellular Localisation | Mitochondrion |
| Alternative Antibody Names | Anti-Succinate--Coa Ligase Gdp-Forming Subunit Beta - Mitochondrial antibodyAnti-Gtp-Specific Succinyl-Coa Synthetase Subunit Beta antibodyAnti-G-Scs antibodyAnti-Gtpscs antibodyAnti-Succinyl-Coa Synthetase Beta-G Chain antibodyAnti-Scs-Betag antibodyAnti-SUCLG2 antibody |
Information sourced from Uniprot.org