Confocal imaging of paraffin-embedded Mouse brain tissue using Talin 2 Rabbit monoclonal antibody (STJ11103151, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-Talin 2 antibody (350-450) [S1MR] (STJ11103151)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11103151
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General Information

Short DescriptionRabbit monoclonal Talin 2 (350-450) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC-P/IF/ICC/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDS1MR
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:1000-1:6000
IHC-P:1:500-1:2000
IF/ICC:1:100-1:400
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolTLN2
Gene ID83660
Uniprot IDTLN2_HUMAN
Immunogen Region350-450
Immunogen SequenceVLQEWPLTTVKRWAASPKSF TLDFGEYQESYYSVQTTEGE QISQLIAGYIDIILKKKQSK DRFGLEGDEESTMLEESVSP KKSTILQQQFNRTGKAEHGS V
SpecificityA synthetic peptide corresponding to a sequence within amino acids 350-450 of human Talin 2 (Q9Y4G6).

Additional Info

Function As a major component of focal adhesion plaques that links integrin to the actin cytoskeleton, may play an important role in cell adhesion. Recruits PIP5K1C to focal adhesion plaques and strongly activates its kinase activity.
Protein Name Talin-2
Database Links
Cellular Localisation Cytoplasm
Cell Junction
Focal Adhesion
Synapse
Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cytoskeleton
Focal Adhesion Plaques And Synapses
Alternative Antibody Names Anti-Talin-2 antibody
Anti-TLN2 antibody
Anti-KIAA0320 antibody

Information sourced from Uniprot.org

Citations

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