• Biotinylated STJ72639 (1µg/ml) staining of Human Thyroid Gland lysate (35µg protein in RIPA buffer) , exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-thyroid peroxidase, Biotinylated antibody (C-Term) {Biotin} (STJ73198)

SKU:
STJ73198-100

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Host: Goat
Applications: Pep-ELISA/WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-thyroid peroxidase, Biotinylated (C-Term) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Biotin
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-1-3µg/ml
ELISA-antibody detection limit dilution 1:32000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: TPO
Gene ID: 7173
Uniprot ID: PERT_HUMAN
Immunogen Region: C-Term
Accession Number: NP_000538.3; NP_783650.1; NP_783652.1; NP_783653.1
Specificity: This antibody is expected to recognize all reported isoforms (NP_000538.3; NP_783650.1; NP_783652.1; NP_783653.1). Reported variants represent identical protein: NP_000538.3, NP_001193673.1 Reported variants represent identical protein: NP_783650.1,
Immunogen Sequence: ELRCGKHQAVGTSPQ.
Function Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4).
Protein Name Thyroid Peroxidase
Tpo
Database Links Reactome: R-HSA-209968
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Isoform 3: Cell Surface
Alternative Antibody Names Anti-Thyroid Peroxidase antibody
Anti-Tpo antibody
Anti-TPO antibody

Information sourced from Uniprot.org

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