Anti-TLE1/2/3/4 antibody (721-770 aa) (STJ98577)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the C-terminal region of human TLE1/TLE2/TLE3/TLE4 at the amino acid range 721-770
General Information
| Short Description | Rabbit polyclonal anti-Transducin-like enhancer protein 1 and Transducin-like enhancer protein 2 and Transducin-like enhancer protein 3 and Transducin-like enhancer protein 4 (721-770 aa) for use in WB, ELISA and IHC in Human, Mouse and Rat samples. |
| Applications | WB/ELISA/IHC |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000IHC-P 1:50-300ELISA 2000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TLE4TLE3709170907089TLE4_HUMANTLE3_HUMANTLE2_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the C-terminal region of human TLE1/TLE2/TLE3/TLE4 at the amino acid range 721-770 |
| Immunogen Region | 721-770 aa |
| Specificity | TLE1/2/3/4 Polyclonal Antibody detects endogenous levels of TLE1/2/3/4 |
Additional Info
| Post Translational Modifications | Phosphorylated, probably by CDK1. The degree of phosphorylation varies throughout the cell cycle, and is highest at the G2/M transition. Becomes hyperphosphorylated in response to cell differentiation and interaction with HES1 or RUNX1. Ubiquitinated by XIAP/BIRC4. |
| Function | Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG. |
| Protein Name | Transducin-Like Enhancer Protein 1E(Sp1 HomologEnhancer Of Split Groucho-Like Protein 1Esg1 |
| Database Links | Reactome: R-HSA-201722Reactome: R-HSA-2122947Reactome: R-HSA-3769402Reactome: R-HSA-4641265 |
| Cellular Localisation | NucleusNuclear And Chromatin-AssociatedDepending On Isoforms And Phosphorylation StatusHyperphosphorylation Decreases The Affinity For Nuclear Components |
| Alternative Antibody Names | Anti-Transducin-Like Enhancer Protein 1 antibodyAnti-E(Sp1 Homolog antibodyAnti-Enhancer Of Split Groucho-Like Protein 1 antibodyAnti-Esg1 antibodyAnti-TLE1 antibody |
Information sourced from Uniprot.org