Host: | Rabbit |
Applications: | WB |
Reactivity: | Human/Mouse |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description : | Rabbit polyclonal antibody anti-E3 ubiquitin-protein ligase TRAIP (399-449 aa) is suitable for use in Western Blot research applications. |
Clonality : | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Concentration: | 1 mg/mL |
Dilution Range: | WB 1:500-2000 |
Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Gene Symbol: | TRAIP |
Gene ID: | 10293 |
Uniprot ID: | TRAIP_HUMAN |
Immunogen Region: | 399-449 aa |
Specificity: | This antibody detects endogenous levels of TRAIP at Human/Mouse |
Immunogen: | Synthesized peptide derived from the human TRAIP at the amino acid range 399-449 |
Function | E3 ubiquitin ligase required to protect genome stability in response to replication stress. Acts as a key regulator of interstrand cross-link repair, which takes place when both strands of duplex DNA are covalently tethered together, thereby blocking replication and transcription. Controls the choice between the two pathways of replication-coupled interstrand-cross-link repair by mediating ubiquitination of MCM7 subunit of the CMG helicase complex. Short ubiquitin chains on MCM7 promote recruitment of DNA glycosylase NEIL3. If the interstrand cross-link cannot be cleaved by NEIL3, the ubiquitin chains continue to grow on MCM7, promoting the unloading of the CMG helicase complex by the VCP/p97 ATPase, enabling the Fanconi anemia DNA repair pathway. Only catalyzes ubiquitination of MCM7 when forks converge. Also involved in the repair of covalent DNA-protein cross-links (DPCs) during DNA synthesis: promotes ubiquitination of DPCs, leading to their degradation by the proteasome. Has also been proposed to play a role in promoting translesion synthesis by mediating the assembly of 'Lys-63'-linked poly-ubiquitin chains on the Y-family polymerase POLN in order to facilitate bypass of DNA lesions and preserve genomic integrity. The function in translesion synthesis is however controversial. Acts as a regulator of the spindle assembly checkpoint. Also acts as a negative regulator of innate immune signaling by inhibiting activation of NF-kappa-B mediated by TNF. Negatively regulates TLR3/4- and RIG-I-mediated IRF3 activation and subsequent IFNB1 production and cellular antiviral response by promoting 'Lys-48'-linked polyubiquitination of TNK1 leading to its proteasomal degradation. |
Protein Name | E3 Ubiquitin-Protein Ligase Traip Ring Finger Protein 206 Traf-Interacting Protein |
Database Links | Reactome: R-HSA-983168 |
Cellular Localisation | Nucleus Nucleoplasm Nucleolus Chromosome Cytoplasm Perinuclear Region In The Nucleus Found In Close Proximity To Pcna Suggesting Localization At Replication Foci Localizes To Dna Damage Sites In Response To Replication Stress |
Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Traip antibody Anti-Ring Finger Protein 206 antibody Anti-Traf-Interacting Protein antibody Anti-TRAIP antibody Anti-RNF206 antibody Anti-TRIP antibody |
Information sourced from Uniprot.org