• Biotinylated STJ71402 (0. 3µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer) , exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-UXT, Biotinylated antibody (C-Term) {Biotin} (STJ73205)

SKU:
STJ73205-100

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Host: Goat
Applications: Pep-ELISA/WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-UXT, Biotinylated (C-Term) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Biotin
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.3-1µg/ml
ELISA-antibody detection limit dilution 1:64000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: UXT
Gene ID: 8409
Uniprot ID: UXT_HUMAN
Immunogen Region: C-Term
Accession Number: NP_705582.1; NP_004173.1
Specificity: This antibody is expected to recognize both reported isoforms (NP_004173.1; NP_705582.1).
Immunogen Sequence: DHRDKVYEQLAKY.
Post Translational Modifications Isoform 2: Ubiquitinated by E3 ubiquitin-protein ligase complex containing FBXO7.leading to proteasomal degradation.
Function Involved in gene transcription regulation. Acts in concert with the corepressor URI1 to regulate androgen receptor AR-mediated transcription. Together with URI1, associates with chromatin to the NKX3-1 promoter region. Negatively regulates the transcriptional activity of the estrogen receptor ESR1 by inducing its translocation into the cytoplasm. May act as nuclear chaperone that facilitates the formation of the NF-kappa-B enhanceosome and thus positively regulates NF-kappa-B transcription activity. Potential component of mitochondrial-associated LRPPRC, a multidomain organizer that potentially integrates mitochondria and the microtubular cytoskeleton with chromosome remodeling. Increasing concentrations of UXT contributes to progressive aggregation of mitochondria and cell death potentially through its association with LRPPRC. Suppresses cell transformation and it might mediate this function by interaction and inhibition of the biological activity of cell proliferation and survival stimulatory factors like MECOM. Isoform 1: Plays a role in protecting cells against TNF-alpha-induced apoptosis by preventing the recruitment of FADD and caspase 8 to the apoptotic complex I, composed of TRADD, TRAF2 and RIPK1/RIP.
Protein Name Protein Uxt
Androgen Receptor Trapped Clone 27 Protein
Art-27
Ubiquitously Expressed Transcript Protein
Database Links Reactome: R-HSA-8953750
Cellular Localisation Isoform 1: Cytoplasm
Isoform 2: Nucleus
Cytoplasm
Nucleus
Cytoskeleton
Microtubule Organizing Center
Centrosome
Spindle Pole
Predominantly Localizes To The Nucleus
Localizes To Spindle Pole During Mitosis
Alternative Antibody Names Anti-Protein Uxt antibody
Anti-Androgen Receptor Trapped Clone 27 Protein antibody
Anti-Art-27 antibody
Anti-Ubiquitously Expressed Transcript Protein antibody
Anti-UXT antibody
Anti-HSPC024 antibody

Information sourced from Uniprot.org

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