![Immunoprecipitation of YTHDC2 in 200 µg extracts from 293T cells using 0. 5 µg [KD Validated] YTHDC2 Rabbit polyclonal antibody (STJ11105298). Western blot analysis was performed using [KD Validated] YTHDC2 Rabbit polyclonal antibody (STJ11105298) at 1:1000 dilution.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/177942/369971/STJ11105298_1__81591.1713135883.jpg?c=1 "Immunoprecipitation of YTHDC2 in 200 µg extracts from 293T cells using 0. 5 µg [KD Validated] YTHDC2 Rabbit polyclonal antibody (STJ11105298). Western blot analysis was performed using [KD Validated] YTHDC2 Rabbit polyclonal antibody (STJ11105298) at 1:1000 dilution.")
![Immunofluorescence analysis of paraffin-embedded Rat testis using [KD Validated] YTHDC2 Rabbit polyclonal antibody (STJ11105298) at a dilution of 1:150 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/177942/369972/STJ11105298_2__16725.1713135884.jpg?c=1 "Immunofluorescence analysis of paraffin-embedded Rat testis using [KD Validated] YTHDC2 Rabbit polyclonal antibody (STJ11105298) at a dilution of 1:150 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunofluorescence analysis of paraffin-embedded Mouse testis using [KD Validated] YTHDC2 Rabbit polyclonal antibody (STJ11105298) at a dilution of 1:150 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/177942/369973/STJ11105298_3__54241.1713135885.jpg?c=1 "Immunofluorescence analysis of paraffin-embedded Mouse testis using [KD Validated] YTHDC2 Rabbit polyclonal antibody (STJ11105298) at a dilution of 1:150 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western blot analysis of lysates from wild type (WT) and YTHDC2 knockdown (KD) HeLa cells using [KD Validated] YTHDC2 Rabbit polyclonal antibody (STJ11105298) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/177942/369974/STJ11105298_4__13907.1713135885.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and YTHDC2 knockdown (KD) HeLa cells using [KD Validated] YTHDC2 Rabbit polyclonal antibody (STJ11105298) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 30s.")
![Western blot analysis of various lysates using [KD Validated] YTHDC2 Rabbit polyclonal antibody (STJ11105298) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/177942/369975/STJ11105298_5__40917.1713135886.jpg?c=1 "Western blot analysis of various lysates using [KD Validated] YTHDC2 Rabbit polyclonal antibody (STJ11105298) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 30s.")
![Immunoprecipitation of YTHDC2 in 200 µg extracts from 293T cells using 0. 5 µg [KD Validated] YTHDC2 Rabbit polyclonal antibody (STJ11105298). Western blot analysis was performed using [KD Validated] YTHDC2 Rabbit polyclonal antibody (STJ11105298) at 1:1000 dilution.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/177942/369971/STJ11105298_1__81591.1713135883.jpg?c=1 "Immunoprecipitation of YTHDC2 in 200 µg extracts from 293T cells using 0. 5 µg [KD Validated] YTHDC2 Rabbit polyclonal antibody (STJ11105298). Western blot analysis was performed using [KD Validated] YTHDC2 Rabbit polyclonal antibody (STJ11105298) at 1:1000 dilution.")
Anti-YTHDC2 antibody (1286-1430) (STJ11105298)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal 3 5 Rna Helicase Ythdc2 (1286-1430) antibody for use in WB, IF, ICC, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | YTHDC2 |
| Gene ID | 64848 |
| Uniprot ID | YTDC2_HUMAN |
| Immunogen Region | 1286-1430 |
| Immunogen Sequence | MPVRYFIMKSSNLRNLEISQ QKGIWSTTPSNERKLNRAFW ESSIVYLVFSVQGSGHFQGF SRMSSEIGREKSQDWGSAGL GGVFKVEWIRKESLPFQFAH HLLNPWNDNKKVQISRDGQE LEPLVGEQLLQLWERLPLGE KNTTD |
| Specificity | Recombinant Protein corresponding to a sequence within amino acids 1286-1430 of human YTHDC2 (NP_073739.3). |
Additional Info
| Tissue Specificity | Expressed in testis. Not detected in spermatogonia next to the tubule wall but is strongly expressed in spermatocytes, suggesting that it is up-regulated in germ cells upon entry into meiosis. |
| Function | 3'-5' RNA helicase that plays a key role in the male and female germline by promoting transition from mitotic to meiotic divisions in stem cells. Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, a modification present at internal sites of mRNAs and some non-coding RNAs that plays a role in the efficiency of RNA processing and stability. Essential for ensuring a successful progression of the meiotic program in the germline by regulating the level of m6A-containing RNAs. Acts by binding and promoting degradation of m6A-containing mRNAs: the 3'-5' RNA helicase activity is required for this process and RNA degradation may be mediated by XRN1 exoribonuclease. Required for both spermatogenesis and oogenesis. |
| Protein Name | 3'-5' Rna Helicase Ythdc2Yth Domain-Containing Protein 2Hythdc2 |
| Database Links | |
| Cellular Localisation | CytoplasmPerinuclear Region |
| Alternative Antibody Names | Anti-3'-5' Rna Helicase Ythdc2 antibodyAnti-Yth Domain-Containing Protein 2 antibodyAnti-Hythdc2 antibodyAnti-YTHDC2 antibody |
Information sourced from Uniprot.org