St John's Laboratory's Enzyme-Linked Immunosorbent Assay (ELISA) kits deliver high-sensitivity detection and accurate quantification of target proteins, antibodies, and biomarkers across diverse biological samples. From cytokine profiling to disease marker validation, our ELISA kits provide reproducible, publication-quality results in high-throughput formats.
Detect even low-abundance targets with confidence. Our kits deliver picogram-level sensitivity with minimal cross-reactivity, ensuring reliable quantification in complex samples.
Standardized protocols and quality-controlled reagents guarantee consistent, batch-to-batch reproducibility for reliable comparative studies.
Choose from direct, indirect, sandwich, and competitive ELISA formats to match your experimental needs and sample types.
Requires only a standard microplate reader—no specialised equipment needed. Accessible, economical, and perfect for high-throughput screening.
Different research questions require different assay architectures. Select the optimal format for your application:
Fastest, simplest format
Flexible, signal-amplified detection
Maximum specificity & sensitivity
Small antigens & inhibition assays
ELISA detection relies on enzyme-conjugated antibodies producing measurable colour changes through chromogenic substrates. Our kits utilise industry-standard enzymes for robust, reliable signal generation:
High catalytic activity with TMB or OPD substrates. Excellent sensitivity with low background in most sample types.
Stable signal with pNPP substrate. Ideal for long incubations and resistant to inhibitors in serum or tissue lysates.
Whether using direct labelling or secondary amplification, the chromogenic substrate generates a colour intensity directly proportional to antigen concentration, measured spectrophotometrically for precise quantification.
High-throughput ELISA workflow supporting reproducible biomarker quantification
| Research Area | Target | Product | SKU | Format |
|---|---|---|---|---|
| Immune Response | Lactoferrin (LTF) | Human Lactoferrin ELISA Kit | STJE0019609 | Sandwich |
| Drug Transport | ABCC4 | Human ABCC4 ELISA Kit | STJE0013198 | Sandwich |
| Bone Metabolism | P1NP | Human P1NP ELISA Kit | STJE0002042 | Sandwich |
| Neurobiology | Serotonin (5-HT) | Serotonin ELISA Kit | STJE0006586 | Competitive |
In a study by Dang et al. (Mucosal Immunol, 2023), St John's Lactoferrin ELISA Kit enabled sensitive quantification of extracellular lactoferrin in bronchoalveolar lavage fluid (BALF).
The kit provided precise monitoring of lactoferrin—a key marker of neutrophil activation—revealing how butyrate modulates neutrophil function during infection. Combined with qPCR and immunoblotting, this multi-technique approach advanced understanding of host-pathogen interactions and mucosal immunity.
This demonstrates the utility of our ELISA kits in complex, publication-quality immunological research.
St John's Laboratory offers comprehensive reagents for building custom ELISA assays, from initial screening to fully validated sandwich immunoassays.
| Antibody Type | Characteristics | Best For |
|---|---|---|
| Monoclonal Antibodies | Single epitope recognition, high specificity, excellent batch consistency | Detection antibodies, discriminating closely related targets |
| Polyclonal Antibodies | Multiple epitope recognition, robust signal amplification | Capture antibodies, maximising antigen binding in sandwich assays |
| Recombinant Antibodies | Engineered sequences, superior lot-to-lot consistency, optimised binding | Long-term studies requiring reproducibility, GMP-compatible workflows |
Developing sandwich ELISA requires antibody pairs recognising distinct, non-overlapping epitopes on the target. This ensures simultaneous binding of capture and detection antibodies without competition, maximising sensitivity and dynamic range.
Our validated antibody pairs are pre-screened for compatibility, saving you optimisation time and ensuring robust assay performance from the start.
How to Get the Most Out of Your ELISA
Troubleshooting tips, protocol refinement, and best practices for maximising assay performance.
Choosing Between Sandwich and Competitive ELISA
Decision framework for selecting the right format based on antigen size and sample complexity.
Our technical team can recommend the optimal format, antibody pair, or detection strategy for your research.
Contact Our ELISA SpecialistsFor Research Use Only - All products are intended for research purposes and are not for use in diagnostic procedures