FITC-Conjugated Secondary Antibodies
Fluorescein isothiocyanate conjugates for flow cytometry and immunomicroscopy
FITC is a fluorophore conjugated directly onto the secondary antibody, absorbing ultraviolet or blue light and emitting a yellow-green light when excited. This makes it a standard green-channel choice for immunofluorescence, immunocytochemistry, immunohistochemistry and flow cytometry work, and it pairs well with DAPI nuclear counterstains and red-channel dyes in multi-colour panels. Our FITC range covers donkey, goat, chicken and mouse host secondaries across mouse, rabbit, rat, chicken, human, goat, HBV antigens and other reactivities.
Browse FITC Secondary AntibodiesTwo of Our Most-Used FITC Secondaries
Goat anti-mouse and goat anti-rabbit IgG (H+L) are the two reactivities most immunofluorescence and confocal protocols reach for first, since a large share of primary antibodies on the market are raised in mouse or rabbit. Both are validated with real staining data below.
Goat Anti-Mouse IgG (H+L) Antibody {FITC}
Shown here detecting an Alpha-Tubulin mouse monoclonal in confocal imaging of HeLa cells, with DAPI marking the nucleus. Validated for IF, ICC and flow cytometry.
View product →
Goat Anti-Rabbit IgG Heavy & Light Chain Antibody {FITC}
Cited in a 2019 Frontiers in Neuroscience study on neuronal apoptosis in an oxygen-glucose deprivation model1. Validated for WB, IHC, IF and ELISA.
View product →Anti-Rabbit IgG FITC Secondary Antibodies
Rabbit polyclonals make up a large share of the primary antibody market, so an anti-rabbit FITC secondary is one of the most frequently stocked reagents in an immunofluorescence lab. We supply this reactivity from both a donkey and a goat host, giving a choice of host species where a multi-colour panel needs to avoid cross-reactivity with another secondary already in use.
Anti-Rat and Anti-Chicken FITC Secondary Antibodies
Beyond mouse and rabbit, our FITC range covers rat and chicken IgY reactivities, both goat-host and validated with real staining or flow cytometry data. Rat-raised primaries are common in neuroscience work, and a chicken-host or anti-chicken secondary suits panels built around a chicken IgY primary or an avian model.
More Hosts and Reactivities in the FITC Range
Beyond the validated conjugates above, our FITC range also covers donkey anti-goat, chicken-host secondaries against mouse and rabbit IgG, rabbit-host secondaries against mouse and rat IgG, and an antigen-specific conjugate against hepatitis B surface antigen. These extend the choice of host and reactivity where a panel needs a species combination not covered by the products above.
Monoclonal, antigen-specific for hepatitis B surface antigen.
Browse Other Secondary Antibody Conjugates
Where a different channel, an electron-dense label or a chromogenic readout suits the assay better, our secondary antibody range is also available in Alexa Fluor, DyLight, HRP, Biotin and gold-labelled formats. Select a conjugation type below to browse that part of the range.
Photostable, pH-stable fluorescent conjugates spanning 350 to 790 nm.
Browse Alexa Fluor →Fluorescent conjugates for multi-colour panels, including near-infrared channels.
Browse DyLight →Chromogenic and chemiluminescent conjugates for western blot and ELISA.
Browse HRP →Biotin conjugates for streptavidin and avidin-based detection systems.
Browse Biotinylated →Colloidal gold conjugates for electron microscopy and immunoassay development.
Browse Gold-Labelled →Learning and Support
Matching FITC to your instrument and panel
FITC is excited around 495 nm and emits around 519 nm, in the green channel most fluorescence microscopes and flow cytometers are set up to read by default. It pairs well with a blue DAPI nuclear stain and a red-channel dye such as a DyLight 550 or 594 conjugate in the same multi-colour panel, though it can photobleach faster than some newer dyes under sustained excitation.
Choosing a host and reactivity
Where a panel already uses one host species secondary in another channel, choosing a different host for the FITC conjugate helps avoid cross-reactivity. Not sure which host or reactivity fits your panel? Talk to us and our team can help you select the right products.
Looking for a Specific Reactivity or Host?
Browse the full range of FITC-conjugated secondary antibodies, or contact us if a specific host or reactivity is not listed.
Browse FITC Secondary AntibodiesReferences
- Zhang Z, et al. miRNA-7a-2-3p inhibits neuronal apoptosis in oxygen-glucose deprivation (OGD) model. Front Neurosci. 2019;13, as cited on the product datasheet for STJ99668.