| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This human ANG kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
ANG |
| Gene ID: |
283 |
| Uniprot ID: |
ANGI_HUMAN |
| Specificity: |
This kit recognizes Human ANG in samples. No significant cross-reactivity or interference between Human ANG and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed predominantly in the liver. Also detected in endothelial cells and spinal cord neurons. |
| Function | Ribonuclease that cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs) which inhibit protein synthesis and triggers the assembly of stress granules (SGs). Binds to actin on the surface of endothelial cells.once bound, angiogenin is endocytosed and translocated to the nucleus. Stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA. Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo. |
| Protein Name | AngiogeninRibonuclease 5Rnase 5 |
| Database Links | Reactome: R-HSA-418990Reactome: R-HSA-9708296 |
| Cellular Localisation | Cytoplasmic VesicleSecretory Vesicle LumenSecretedNucleusNucleolusRapidly Endocytosed By Target Cells And Translocated To The Nucleus Where It Accumulates In The Nucleolus And Binds To Dna |
| Alternative CLIA Names | Angiogenin CLIA kitRibonuclease 5 CLIA kitRnase 5 CLIA kitANG CLIA kitRNASE5 CLIA kit |
| Specificity | This kit recognizes Human ANG in samples. No significant cross-reactivity or interference between Human ANG and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 96-111 | 103 |
| EDTA plasma(n=8) | 87-100 | 94 |
| Cell culture media(n=8) | 95-110 | 103 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 107.95 | 252.88 | 924.50 | 113.60 | 240.62 | 994.18 |
| Standard deviation | 9.51 | 23.52 | 83.02 | 10.41 | 23.58 | 81.03 |
| CV (%) | 8.81 | 9.30 | 8.98 | 9.16 | 9.80 | 8.15 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
