• Human CCL11 (Eotaxin) Sandwich ELISA (STJE0001928)

Human CCL11 (Eotaxin) Sandwich ELISA (STJE0001928)

SKU:
STJE0001928

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63~1000pg/mL
Short Description: This CCL11 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: CCL11
Gene ID: 6356
Uniprot ID: CCL11_HUMAN
Specificity: This kit recognizes Human ECF/CCL11 in samples. No significant cross-reactivity or interference between Human ECF/CCL11 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions. Binds to CCR3.
Protein Name Eotaxin
C-C Motif Chemokine 11
Eosinophil Chemotactic Protein
Small-Inducible Cytokine A11
Database Links Reactome: R-HSA-380108
Reactome: R-HSA-6785807
Cellular Localisation Secreted
Alternative ELISA Names Eotaxin ELISA kit
C-C Motif Chemokine 11 ELISA kit
Eosinophil Chemotactic Protein ELISA kit
Small-Inducible Cytokine A11 ELISA kit
CCL11 ELISA kit
SCYA11 ELISA kit
Specificity This kit recognizes Human ECF/CCL11 in samples. No significant cross-reactivity or interference between Human ECF/CCL11 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 96-108 101
EDTA plasma (n=8) 85-97 91
Cell culture media (n=8) 91-106 98

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 51.30 124.70 336.30 46.80 127.70 341.40
Standard deviation 2.60 5.10 10.10 2.90 6.40 16.40
CV (%) 5.07 4.09 3.00 6.20 5.01 4.80
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance