• Human GFAP (Glial fibrillary acidic protein) Sandwich ELISA (STJE0004105)

Human GFAP (Glial fibrillary acidic protein) Sandwich ELISA (STJE0004105)

SKU:
STJE0004105

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Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63~1000pg/mL
Short Description: This GFAP Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: GFAP
Gene ID: 2670
Uniprot ID: GFAP_HUMAN
Specificity: This kit recognizes Human GFAP in samples. No significant cross-reactivity or interference between Human GFAP and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Phosphorylated by PKN1.
Function GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Protein Name Glial Fibrillary Acidic Protein
Gfap
Database Links Reactome: R-HSA-1251985
Reactome: R-HSA-9613829
Cellular Localisation Cytoplasm
Associated With Intermediate Filaments
Alternative ELISA Names Glial Fibrillary Acidic Protein ELISA kit
Gfap ELISA kit
GFAP ELISA kit
Specificity This kit recognizes Human GFAP in samples. No significant cross-reactivity or interference between Human GFAP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-96 91
EDTA plasma (n=8) 93-104 99
Cell culture media (n=8) 101-107 104

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 41.27 118.65 432.44 44.06 112.01 408.43
Standard deviation 2.46 5.54 19.98 2.41 5.35 22.26
CV (%) 5.96 4.67 4.62 5.47 4.78 5.45
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance