Mouse Apolipoprotein E/APOE protein (Recombinant) (C-His) (STJP002645)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Mouse ApoE/APOE/Apolipoprotein E Protein is produced by Human cells expression system. The target protein is expressed with sequence (Glu19-Gln311) of mouse ApoE/APOE/Apolipoprotein E (Accession #P08226) fused with a 6×His tag at the C-te
General Information
| Short Description | Recombinant-Mouse Apolipoprotein E/APOE-C-His protein was developed in hek293 cells using the region Glu19-Gln311. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by its binding ability in a functional ELISA. Immobilized Mouse APOE at 5 Mu g/mL (100 Mu L/well) can bind Mouse TREM2 with a linear range of 0.16-1.5 Mu g/mL. |
| Determination Method | < 1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | Apoe |
| Gene ID | 11816 |
| Uniprot ID | APOE_MOUSE |
| Immunogen | Recombinant Mouse ApoE/APOE/Apolipoprotein E Protein is produced by Human cells expression system. The target protein is expressed with sequence (Glu19-Gln311) of mouse ApoE/APOE/Apolipoprotein E (Accession #P08226) fused with a 6×His tag at the C-te |
| Immunogen Region | Glu19-Gln311 |
| Immunogen Sequence | EGEPEVTDQLEWQSNQPWEQ ALNRFWDYLRWVQTLSDQVQ EELQSSQVTQELTALMEDTM TEVKAYKKELEEQLGPVAEE TRARLGKEVQAAQARLGADM EDLRNRLGQYRNEVHTMLGQ STEEIRARLSTHLRKMRKRL MRDAEDLQKRLAVYKAGARE GAERGVSAIRERLGPLVEQG RQRTANLGAGAAQPLRDRAQ AFGDRIRGRLEEVGNQARDR LEEVREHMEEVRSKMEEQT |
Additional Info
| Function | APOE is an apolipoprotein, a protein associating with lipid particles, that mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids. APOE is a core component of plasma lipoproteins and is involved in their production, conversion and clearance. Apolipoproteins are amphipathic molecules that interact both with lipids of the lipoprotein particle core and the aqueous environment of the plasma. As such, APOE associates with chylomicrons, chylomicron remnants, very low density lipoproteins (VLDL) and intermediate density lipoproteins (IDL) but shows a preferential binding to high-density lipoproteins (HDL). It also binds a wide range of cellular receptors including the LDL receptor/LDLR and the very low-density lipoprotein receptor/VLDLR that mediate the cellular uptake of the APOE-containing lipoprotein particles. Finally, APOE also has a heparin-binding activity and binds heparan-sulfate proteoglycans on the surface of cells, a property that supports the capture and the receptor-mediated uptake of APOE-containing lipoproteins by cells. |
| Protein Name | Apolipoprotein EApo-E |
| Database Links | Reactome: R-MMU-3000480Reactome: -MMU-381426Reactome: -MMU-8957275Reactome: -MMU-8963888Reactome: -MMU-8963901Reactome: -MMU-8964026Reactome: -MMU-8964058Reactome: -MMU-975634 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular MatrixExtracellular VesicleEndosomeMultivesicular BodyIn The PlasmaApoe Is Associated With ChylomicronsChylomicrons RemnantsVldlLdl And Hdl LipoproteinsLipid Poor Oligomeric Apoe Is Associated With The Extracellular Matrix In A Calcium- And Heparan-Sulfate Proteoglycans-Dependent MannerLipidation Induces The Release From The Extracellular MatrixColocalizes With Cd63 And Pmel At Exosomes And In Intraluminal Vesicles Within Multivesicular Endosomes |
| Alternative Protein Names | Apolipoprotein E proteinApo-E proteinApoe protein |
Information sourced from Uniprot.org