| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.09ng/mL |
| Detection Limit: |
0.16~10ng/mL |
| Short Description: |
This Casp9 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Casp9 |
| Gene ID: |
12371 |
| Uniprot ID: |
CASP9_MOUSE |
| Specificity: |
This kit recognizes Mouse CASP9 in samples. No significant cross-reactivity or interference between Mouse CASP9 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates effector caspases caspase-3 (CASP3) or caspase-7 (CASP7). Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). |
| Protein Name | Caspase-9Casp-9Apoptotic Protease Mch-6Apoptotic Protease-Activating Factor 3Apaf-3Ice-Like Apoptotic Protease 6Ice-Lap6 Cleaved Into - Caspase-9 Subunit P35 - Caspase-9 Subunit P10 |
| Database Links | Reactome: R-MMU-111458Reactome: -MMU-111459Reactome: -MMU-168638Reactome: -MMU-198323Reactome: -MMU-418889Reactome: -MMU-9627069 |
| Alternative ELISA Names | Caspase-9 ELISA kitCasp-9 ELISA kitApoptotic Protease Mch-6 ELISA kitApoptotic Protease-Activating Factor 3 ELISA kitApaf-3 ELISA kitIce-Like Apoptotic Protease 6 ELISA kitIce-Lap6 Cleaved Into - Caspase-9 Subunit P35 - Caspase-9 Subunit P10 ELISA kitCasp9 ELISA kitMch6 ELISA kit |
| Specificity | This kit recognizes Mouse CASP9 in samples. No significant cross-reactivity or interference between Mouse CASP9 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 87-97 | 92 |
| EDTA plasma (n=8) | 87-99 | 94 |
| Cell culture media (n=8) | 91-106 | 98 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.54 | 1.28 | 4.04 | 0.57 | 1.23 | 4.02 |
| Standard deviation | 0.03 | 0.05 | 0.15 | 0.04 | 0.07 | 0.13 |
| CV (%) | 5.56 | 3.91 | 3.71 | 7.02 | 5.69 | 3.23 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
