• Rat bACE1 (Beta-Site APP Cleaving Enzyme 1) Sandwich ELISA Kit Ready-To-Use (STJE0013796)

Rat bACE1 (Beta-Site APP Cleaving Enzyme 1) Sandwich ELISA Kit Ready-To-Use (STJE0013796)

SKU:
STJE0013796

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Applications: ELISA
Reactivity: Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.062ng/mL
Detection Limit: 0.156-10ng/mL
Short Description: This bACE1 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 3 hrs
Gene Symbol: Bace1
Gene ID: 29392
Uniprot ID: BACE1_RAT
Immunogen Region: Ready-To-Use
Sample Type: serum, plasma, cerebrospinal fluid or other biological fluids.
Tissue Specificity
Post Translational Modifications Palmitoylation mediates lipid raft localization. Acetylated in the endoplasmic reticulum at Lys-126, Lys-275, Lys-279, Lys-285, Lys-299, Lys-300 and Lys-307. Acetylation by NAT8 and NAT8B is transient and deacetylation probably occurs in the Golgi. Acetylation regulates the maturation, the transport to the plasma membrane, the stability and the expression of the protein. Ubiquitinated at Lys-501, ubiquitination leads to lysosomal degradation. Monoubiquitinated and 'Lys-63'-linked polyubitinated. Deubiquitnated by USP8.inhibits lysosomal degradation. Phosphorylation at Ser-498 is required for interaction with GGA1 and retrograded transport from endosomal compartments to the trans-Golgi network. Non-phosphorylated BACE1 enters a direct recycling route to the cell surface. N-Glycosylated. Addition of a bisecting N-acetylglucosamine by MGAT3 blocks lysosomal targeting, further degradation and is required for maintaining stability under stress conditions.
Function Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. Cleaves CHL1.
Protein Name Beta-Secretase 1
Aspartyl Protease 2
Asp2
Asp 2
Beta-Site Amyloid Precursor Protein Cleaving Enzyme 1
Beta-Site App Cleaving Enzyme 1
Memapsin-2
Membrane-Associated Aspartic Protease 2
Database Links
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Golgi Apparatus
Trans-Golgi Network
Endoplasmic Reticulum
Endosome
Cell Surface
Cytoplasmic Vesicle Membrane
Membrane Raft
Lysosome
Late Endosome
Early Endosome
Recycling Endosome
Cell Projection
Axon
Dendrite
Predominantly Localized To The Later Golgi/Trans-Golgi Network (Tgn) And Minimally Detectable In The Early Golgi Compartments
A Small Portion Is Also Found In The Endoplasmic Reticulum
Endosomes And On The Cell Surface
Colocalization With App In Early Endosomes Is Due To Addition Of Bisecting N-Acetylglucosamine Wich Blocks Targeting To Late Endosomes And Lysosomes
Retrogradly Transported From Endosomal Compartments To The Trans-Golgi Network In A Phosphorylation- And Gga1- Dependent Manner
Alternative ELISA Names Beta-Secretase 1 ELISA kit
Aspartyl Protease 2 ELISA kit
Asp2 ELISA kit
Asp 2 ELISA kit
Beta-Site Amyloid Precursor Protein Cleaving Enzyme 1 ELISA kit
Beta-Site App Cleaving Enzyme 1 ELISA kit
Memapsin-2 ELISA kit
Membrane-Associated Aspartic Protease 2 ELISA kit
Bace1 ELISA kit
Bace ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance