• Rat DLG4 (Discs, Large Homolog 4) Sandwich ELISA Kit Ready-To-Use (STJE0014832)

Rat DLG4 (Discs, Large Homolog 4) Sandwich ELISA Kit Ready-To-Use (STJE0014832)

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STJE0014832

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Applications: ELISA
Reactivity: Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.112ng/mL
Detection Limit: 0.312-20ng/mL
Short Description: This DLG4 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 3 hrs
Gene Symbol: Dlg4
Gene ID: 29495
Uniprot ID: DLG4_RAT
Immunogen Region: Ready-To-Use
Sample Type: tissue homogenates, cell lysates or other biological fluids.
Tissue Specificity Expressed in brain (at protein level). Detected in juxtaparanodal zones in the central nervous system and at nerve terminal plexuses of basket cells in the cerebellum. Expressed in cerebrum. Expressed in hippocampal neurons (at protein level). Isoform 1 and isoform 2: highly expressed in cerebellum, cortex, hippocampus, and corpus striatum.
Post Translational Modifications Palmitoylated. Palmitoylation is required for targeting to postsynaptic density, plasma membrane and synapses. Palmitoylation by ZDHHC2 occurs when the synaptic activity decreases and induces DLG4 synaptic clustering. Palmitoylation by ZDHHC15 regulates trafficking to the postsynaptic density and function in synaptogenesis. Palmitoylation may play a role in glutamate receptor GRIA1 synapse clustering. Depalmitoylated by ABHD17A and ABHD17B and to a lesser extent by ABHD17C, ABHD12, ABHD13, LYPLA1 and LYPLA2. Undergoes rapid synaptic palmitoylation/depalmitoylation cycle during neuronal development which slows down in mature neurons. Ubiquitinated by MDM2 in response to NMDA receptor activation, leading to proteasome-mediated degradation of DLG4 which is required for AMPA receptor endocytosis.
Function Postsynaptic scaffolding protein that plays a critical role in synaptogenesis and synaptic plasticity by providing a platform for the postsynaptic clustering of crucial synaptic proteins. Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B. Also regulates AMPA-type glutamate receptor (AMPAR) immobilization at postsynaptic density keeping the channels in an activated state in the presence of glutamate and preventing synaptic depression.Under basal conditions, cooperates with FYN to stabilize palmitoyltransferase ZDHHC5 at the synaptic membrane through FYN-mediated phosphorylation of ZDHHC5 and its subsequent inhibition of association with endocytic proteins.
Protein Name Disks Large Homolog 4
Postsynaptic Density Protein 95
Psd-95
Synapse-Associated Protein 90
Sap-90
Sap90
Database Links Reactome: R-RNO-399719
Reactome: -RNO-438066
Reactome: -RNO-451308
Reactome: -RNO-5625900
Reactome: -RNO-5673001
Reactome: -RNO-5682910
Reactome: -RNO-6794361
Reactome: -RNO-8849932
Cellular Localisation Cell Membrane
Lipid-Anchor
Cytoplasmic Side
Postsynaptic Density
Synapse
Cytoplasm
Cell Projection
Axon
Dendritic Spine
Dendrite
Presynapse
High Levels In Postsynaptic Density Of Neurons In The Forebrain
Also In Presynaptic Region Of Inhibitory Synapses Formed By Cerebellar Basket Cells On Axon Hillocks Of Purkinje Cells
Suppression Of Neuronal Activity Induces Synaptic Accumulation And Clustering Of Dlg4
Alternative ELISA Names Disks Large Homolog 4 ELISA kit
Postsynaptic Density Protein 95 ELISA kit
Psd-95 ELISA kit
Synapse-Associated Protein 90 ELISA kit
Sap-90 ELISA kit
Sap90 ELISA kit
Dlg4 ELISA kit
Dlgh4 ELISA kit
Psd95 ELISA kit
output

Information sourced from Uniprot.org

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