Applications: |
CLIA |
Reactivity: |
Rat |
Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Sensitivity: |
4.69pg/mL |
Detection Limit: |
7.81~500pg/mL |
Short Description: |
This rat GAL kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
Assay Time: |
3.5h |
Detection: |
Chemiluminescence |
Gene Symbol: |
Gal |
Gene ID: |
29141 |
Uniprot ID: |
GALA_RAT |
Specificity: |
This kit recognizes Rat GAL in samples. No significant cross-reactivity or interference between Rat GAL and analogues was observed. |
Sample Type: |
Serum, plasma and other biological fluids |
Function | Endocrine hormone of the central and peripheral nervous systems that binds and activates the G protein-coupled receptors GALR1, GALR2, and GALR3. This small neuropeptide may regulate diverse physiologic functions including contraction of smooth muscle of the gastrointestinal and genitourinary tract, growth hormone and insulin release and adrenal secretion. |
Protein Name | Galanin Peptides Cleaved Into - Galanin - Galanin Message-Associated PeptideGmap |
Database Links | Reactome: R-RNO-375276Reactome: -RNO-418594 |
Cellular Localisation | Secreted |
Alternative CLIA Names | Galanin Peptides Cleaved Into - Galanin - Galanin Message-Associated Peptide CLIA kitGmap CLIA kitGal CLIA kitGaln CLIA kit |
Specificity | This kit recognizes Rat GAL in samples. No significant cross-reactivity or interference between Rat GAL and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item | Specifications | Storage |
Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy | |
Sample Type | Range (%) | Average Recovery (%) |
Serum(n=8) | 92-106 | 99 |
EDTA plasma(n=8) | 96-109 | 103 |
Cell culture media(n=8) | 100-117 | 107 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (pg/mL) | 27.16 | 73.25 | 178.01 | 27.25 | 74.66 | 165.66 |
Standard deviation | 2.72 | 7.22 | 10.88 | 2.76 | 5.86 | 13.30 |
CV (%) | 10.01 | 9.86 | 6.11 | 10.13 | 7.85 | 8.03 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance