Rat IL-1 alpha/Il1a protein (Recombinant) (C-6His) (STJP001915)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Rat IL-1 alpha/Il1a Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ser115-Ser270) of rat IL-1 alpha/Il1a (Accession #) fused with and a 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Rat IL-1 alpha/Il1a-C-6His protein was developed in hek293 cells using the region Ser115-Ser270. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method | < 1 EU/Mu g |
Target Information
| Gene Symbol | Il1a |
| Gene ID | 24493 |
| Uniprot ID | IL1A_RAT |
| Immunogen | Recombinant Rat IL-1 alpha/Il1a Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ser115-Ser270) of rat IL-1 alpha/Il1a (Accession #) fused with and a 6×His tag at the C-terminus. |
| Immunogen Region | Ser115-Ser270 |
| Immunogen Sequence | SAPHSFQNNLRYKLIRIVKQ EFIMNDSLNQNIYVDMDRIH LKAASLNDLQLEVKFDMYAY SSGGDDSKYPVTLKVSNTQL FVSAQGEDKPVLLKEIPETP KLITGSETDLIFFWEKINSK NYFTSAAFPELLIATKEQSQ VHLARGLPSMIDFQIS |
Additional Info
| Function | Cytokine constitutively present intracellularly in nearly all resting non-hematopoietic cells that plays an important role in inflammation and bridges the innate and adaptive immune systems. After binding to its receptor IL1R1 together with its accessory protein IL1RAP, forms the high affinity interleukin-1 receptor complex. Signaling involves the recruitment of adapter molecules such as MYD88, IRAK1 or IRAK4. In turn, mediates the activation of NF-kappa-B and the three MAPK pathways p38, p42/p44 and JNK pathways. Within the cell, acts as an alarmin and cell death results in its liberation in the extracellular space after disruption of the cell membrane to induce inflammation and alert the host to injury or damage. In addition to its role as a danger signal, which occurs when the cytokine is passively released by cell necrosis, directly senses DNA damage and acts as signal for genotoxic stress without loss of cell integrity. |
| Protein Name | Interleukin-1 AlphaIl-1 Alpha |
| Database Links | Reactome: R-RNO-448706Reactome: -RNO-5620971Reactome: -RNO-9020702 |
| Cellular Localisation | NucleusCytoplasmSecretedThe Lack Of A Specific Hydrophobic Segment In The Precursor Sequence Suggests That Il-1 Is Released By Damaged Cells Or Is Secreted By A Mechanism Differing From That Used For Other Secretory ProteinsThe Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10It Results In Protein Translocation From The Cytoplasm Into The Ergic (Endoplasmic Reticulum-Golgi Intermediate Compartment) Followed By Vesicle Entry And SecretionRecruited To Dna Damage Sites And Secreted After Genotoxic Stress |
| Alternative Protein Names | Interleukin-1 Alpha proteinIl-1 Alpha proteinIl1a protein |
Information sourced from Uniprot.org