| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
9.38pg/mL |
| Detection Limit: |
15.63-1000pg/mL |
| Short Description: |
This IL-1R2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Il1r2 |
| Gene ID: |
117022 |
| Uniprot ID: |
IL1R2_RAT |
| Specificity: |
This kit recognizes Rat IL-1R2 in samples. No significant crossreactivity or interference between Rat IL-1R2 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Non-signaling receptor for IL1A, IL1B and IL1RN. Reduces IL1B activities. Serves as a decoy receptor by competitive binding to IL1B and preventing its binding to IL1R1. Also modulates cellular response through non-signaling association with IL1RAP after binding to IL1B. IL1R2 (membrane and secreted forms) preferentially binds IL1B and poorly IL1A and IL1RN. The secreted IL1R2 recruits secreted IL1RAP with high affinity.this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors. |
| Protein Name | Interleukin-1 Receptor Type 2Il-1r-2Il-1rt-2Il-1rt2Cd121 Antigen-Like Family Member BIl-1 Type Ii ReceptorInterleukin-1 Receptor BetaIl-1r-BetaInterleukin-1 Receptor Type IiCd Antigen Cd121b Cleaved Into - Interleukin-1 Receptor Type 2 - Membrane FormMil-1r2Mil-1rii - Interleukin-1 Receptor Type 2 - Soluble FormSil-1r2Sil-1rii |
| Database Links | Reactome: R-RNO-9020702 |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane ProteinCell MembraneSecreted |
| Alternative ELISA Names | Interleukin-1 Receptor Type 2 ELISA kitIl-1r-2 ELISA kitIl-1rt-2 ELISA kitIl-1rt2 ELISA kitCd121 Antigen-Like Family Member B ELISA kitIl-1 Type Ii Receptor ELISA kitInterleukin-1 Receptor Beta ELISA kitIl-1r-Beta ELISA kitInterleukin-1 Receptor Type Ii ELISA kitCd Antigen Cd121b Cleaved Into - Interleukin-1 Receptor Type 2 - Membrane Form ELISA kitMil-1r2 ELISA kitMil-1rii - Interleukin-1 Receptor Type 2 - Soluble Form ELISA kitSil-1r2 ELISA kitSil-1rii ELISA kitIl1r2 ELISA kitIl-1r2 ELISA kitIl1rb ELISA kit |
| Specificity | This kit recognizes Rat IL-1R2 in samples. No significant cross-reactivity or interference between Rat IL-1R2 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 96-110 | 101 |
| EDTA plasma (n=8) | 95-109 | 101 |
| Cell culture media (n=8) | 89-101 | 94 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 50.25 | 101.76 | 487.92 | 47.89 | 91.68 | 472.62 |
| Standard deviation | 2.83 | 4.43 | 18.83 | 2.47 | 5.43 | 19.33 |
| CV (%) | 5.64 | 4.35 | 3.86 | 5.15 | 5.92 | 4.09 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
