• Western blot analysis of extracts of various cell lines, using EEF1A2 antibody (STJ111156) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-EEF1A2 antibody (184-463) (STJ111156)

SKU:
STJ111156

£169.50 - £449.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-EEF1A2 (184-463) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EEF1A2
Gene ID: 1917
Uniprot ID: EF1A2_HUMAN
Immunogen Region: 184-463
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 184-463 of human EEF1A2 (NP_001949.1).
Immunogen Sequence: NPATVPFVPISGWHGDNMLE PSPNMPWFKGWKVERKEGNA SGVSLLEALDTILPPTRPTD KPLRLPLQDVYKIGGIGTVP VGRVETGILRPGMVVTFAPV NITTEVKSVEMHHEALSEAL PGDNVGFNVKNVSVKDIRRG NVCGDSKSDPPQEAAQFTSQ VIILNHPGQISAGYSPVIDC HTAHIACKFAELKEKIDRRS GKKLEDNPKSLKSGDAAIVE MVPGKPMCVESFSQYPPLG
Tissue Specificity Brain, heart, and skeletal muscle.
Post Translational Modifications Trimethylated at Lys-165 by EEF1AKMT3. Mono-, di-, and trimethylated at Lys-36 by EEF1AKMT4.trimethylated form is predominant. Methylation by EEF1AKMT4 contributes to the fine-tuning of translation rates for a subset of tRNAs. Trimethylated at the N-terminus by METTL13. Mono- and dimethylated at Lys-55 by METTL13.dimethylated form is predominant.
Function This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
Protein Name Elongation Factor 1-Alpha 2
Ef-1-Alpha-2
Eukaryotic Elongation Factor 1 A-2
Eef1a-2
Statin-S1
Database Links Reactome: R-HSA-156842
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Elongation Factor 1-Alpha 2 antibody
Anti-Ef-1-Alpha-2 antibody
Anti-Eukaryotic Elongation Factor 1 A-2 antibody
Anti-Eef1a-2 antibody
Anti-Statin-S1 antibody
Anti-EEF1A2 antibody
Anti-EEF1AL antibody
Anti-STN antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance