• Immunohistochemical analysis of paraffin-embedded Human-skin, antibody was diluted at 1:100
  • Immunohistochemical analysis of paraffin-embedded Human-brain, antibody was diluted at 1:100
  • Immunohistochemical analysis of paraffin-embedded Human-skin, antibody was diluted at 1:100

Anti-FZD4 antibody (11-60 aa) (STJ99005)

SKU:
STJ99005

Current Stock:
Host: Rabbit
Applications: IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Frizzled-4 (11-60 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC-P 1:50-200
ELISA 1:10000-20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FZD4
Gene ID: 8322
Uniprot ID: FZD4_HUMAN
Immunogen Region: 11-60 aa
Specificity: The antibody detects endogenous Frizzled-4
Immunogen: Synthetic peptide from the human protein at the amino acid range 11-60
Post Translational Modifications Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.
Function Receptor for Wnt proteins. Most frizzled receptors are coupled to the beta-catenin (CTNNB1) canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin (CTNNB1) and activation of Wnt target genes. Plays a critical role in retinal vascularization by acting as a receptor for Wnt proteins and norrin (NDP). In retina, it can be activated by Wnt protein-binding and also by Wnt-independent signaling via binding of norrin (NDP), promoting in both cases beta-catenin (CTNNB1) accumulation and stimulation of LEF/TCF-mediated transcriptional programs. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
Protein Name Frizzled-4
Fz-4
Hfz4
Fze4
Cd Antigen Cd344
Database Links Reactome: R-HSA-373080
Reactome: R-HSA-4086398
Reactome: R-HSA-4608870
Reactome: R-HSA-4641263
Reactome: R-HSA-5099900
Reactome: R-HSA-5340588
Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Frizzled-4 antibody
Anti-Fz-4 antibody
Anti-Hfz4 antibody
Anti-Fze4 antibody
Anti-Cd Antigen Cd344 antibody
Anti-FZD4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance