• Western blot analysis of extracts of rat skeletal muscle, using GABRA5 antibody (STJ115919) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-GABRA5 antibody (32-259) (STJ115919)

SKU:
STJ115919

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GABRA5 (32-259) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GABRA5
Gene ID: 2558
Uniprot ID: GBRA5_HUMAN
Immunogen Region: 32-259
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 32-259 of human GABRA5 (NP_000801.1).
Immunogen Sequence: QMPTSSVKDETNDNITIFTR ILDGLLDGYDNRLRPGLGER ITQVRTDIYVTSFGPVSDTE MEYTIDVFFRQSWKDERLRF KGPMQRLPLNNLLASKIWTP DTFFHNGKKSIAHNMTTPNK LLRLEDDGTLLYTMRLTISA ECPMQLEDFPMDAHACPLKF GSYAYPNSEVVYVWTNGSTK SVVVAEDGSRLNQYHLMGQT VGTENISTSTGEYTIMTAHF HLKRKIGY
Function Ligand-gated chloride channel subunit which is a component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the brain. May be involved in GABA-A receptor assembly, and GABA-A receptor immobilization and accumulation by gephyrin at the synapse.
Protein Name Gamma-Aminobutyric Acid Receptor Subunit Alpha-5
Gaba(A Receptor Subunit Alpha-5
Database Links Reactome: R-HSA-977443
Cellular Localisation Postsynaptic Cell Membrane
Multi-Pass Membrane Protein
Cell Membrane
Alternative Antibody Names Anti-Gamma-Aminobutyric Acid Receptor Subunit Alpha-5 antibody
Anti-Gaba(A Receptor Subunit Alpha-5 antibody
Anti-GABRA5 antibody

Information sourced from Uniprot.org

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