Anti-Phospho-CAD (T456) antibody

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Product name Anti-Phospho-CAD (T456) antibody
Short Description Rabbit polyclonal against Phospho-CAD (T456)
Description Rabbit polyclonal to Phospho-CAD (T456).
Applications ELISA, IHC
Dilution range IHC 1:100-1:300
ELISA 1:5000
Specificity Phospho-CAD (T456) Polyclonal Antibody detects endogenous levels of CAD protein only when phosphorylated at T456.
Protein Name CAD protein
Includes: Glutamine-dependent carbamoyl-phosphate synthase
Aspartate carbamoyltransferase
Dihydroorotase
Immunogen Synthesized peptide derived from human CAD around the phosphorylation site of T456.
Immunogen Region 400-480 aa
Storage Instruction Store at -20°C, and avoid repeat freeze-thaw cycles.
Note For Research Use Only (RUO).
Validated Application IHC
Host Rabbit
Clonality Polyclonal
Reactivity Human, Mouse
Conjugation Unconjugated
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Isotype IgG
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID 790
Gene Symbol CAD
Molecular Weight 250 kDa
Database Links HGNC:1424
OMIM:114010
Alternative Names CAD protein
Includes: Glutamine-dependent carbamoyl-phosphate synthase
Aspartate carbamoyltransferase
Dihydroorotase
Function This protein is a "fusion" protein encoding four enzymatic activities of the pyrimidine pathway (GATase, CPSase, ATCase and DHOase).
Post-translational Modifications Activated by MAP kinase (Erk1/2) phosphorylation just prior to the S phase of the cell cycle, when the demand for pyrimidine nucleotides is greatest, and down-regulated as the cells emerge from S phase by protein kinase A (PKA) phosphorylation. Phosphorylation at Ser-1859 by RPS6KB1 downstream of MTOR promotes oligomerization and stimulates dihydroorotase activity. Phosphorylation at Ser-1406 reduces sensitivy to feedback inhibition by UTP.
Cellular Localization Cytoplasm Nucleus. Cytosolic and unphosphorylated in resting cells, translocates to the nucleus in response to EGF stimulation, nuclear import promotes optimal cell growth.
Swiss-Prot Key P27708_HUMAN
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