• Immunohistochemistry analysis of PLS1 in paraffin-embedded mouse kidney using PLS1 Rabbit polyclonal antibody (STJ117498) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of PLS1 in paraffin-embedded rat kidney using PLS1 Rabbit polyclonal antibody (STJ117498) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using PLS1 Rabbit polyclonal antibody (STJ117498) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-PLS1 antibody (1-110) (STJ117498)

SKU:
STJ117498

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PLS1 (1-110) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PLS1
Gene ID: 5357
Uniprot ID: PLSI_HUMAN
Immunogen Region: 1-110
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human PLS1 (NP_001165783.1).
Immunogen Sequence: MENSTTTISREELEELQEAF NKIDIDNSGYVSDYELQDLF KEASLPLPGYKVREIVEKIL SVADSNKDGKISFEEFVSLM QELKSKDISKTFRKIINKRE GITAIGGTST
Tissue Specificity In small intestine, colon, and kidney.relatively lower levels of expression are detected in the lung and stomach.
Post Translational Modifications Phosphorylated.
Function Actin-bundling protein. In the inner ear, it is required for stereocilia formation. Mediates liquid packing of actin filaments that is necessary for stereocilia to grow to their proper dimensions.
Protein Name Plastin-1
Intestine-Specific Plastin
I-Plastin
Database Links Reactome: R-HSA-9662360
Reactome: R-HSA-9662361
Cellular Localisation Cytoplasm
Cell Projection
Stereocilium
Alternative Antibody Names Anti-Plastin-1 antibody
Anti-Intestine-Specific Plastin antibody
Anti-I-Plastin antibody
Anti-PLS1 antibody

Information sourced from Uniprot.org

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