• Immunofluorescence analysis of SH-SY5Y cells using DARPP32 Rabbit polyclonal antibody (STJ26213) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using DARPP32 antibody (STJ26213) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-PPP1R1B antibody (1-116) (STJ26213)

SKU:
STJ26213

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PPP1R1B (1-116) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PPP1R1B
Gene ID: 84152
Uniprot ID: PPR1B_HUMAN
Immunogen Region: 1-116
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-116 of human DARPP32 (NP_115568.2).
Immunogen Sequence: MDPKDRKKIQFSVPAPPSQL DPRQVEMIRRRRPTPAMLFR LSEHSSPEEEASPHQRASGE GHHLKSKRPNPCAYTPPSLK AVQRIAESHLQSISNLNENQ ASEEEDELGELRELGY
Post Translational Modifications Dopamine- and cyclic AMP-regulated neuronal phosphoprotein. Phosphorylation of Thr-34 is required for activity.
Function Inhibitor of protein-phosphatase 1.
Protein Name Protein Phosphatase 1 Regulatory Subunit 1b
Darpp-32
Dopamine- And Camp-Regulated Neuronal Phosphoprotein
Database Links Reactome: R-HSA-180024
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Protein Phosphatase 1 Regulatory Subunit 1b antibody
Anti-Darpp-32 antibody
Anti-Dopamine- And Camp-Regulated Neuronal Phosphoprotein antibody
Anti-PPP1R1B antibody
Anti-DARPP32 antibody

Information sourced from Uniprot.org

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