• Western blot analysis of extracts of various cell lines, using RASEF antibody (STJ115816) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-RASEF antibody (200-500) (STJ115816)

SKU:
STJ115816

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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RASEF (200-500) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RASEF
Gene ID: 158158
Uniprot ID: RASEF_HUMAN
Immunogen Region: 200-500
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 200-500 of human RASEF (NP_689786.2).
Immunogen Sequence: MQLSELEEEMDQRIQAAEHK TRKDEKRKAEEALSDLRRQY ETEVGDLQVTIKKLRKLEEQ SKRVSQKEDVAALKKQIYDL SMENQKVKKDLLEAQTNIAF LQSELDALKSDYADQSLNTE RDLEIIRAYTEDRNSLERQI EILQTANRKLHDSNDGLRSA LENSYSKFNRSLHINNISPG NTISRSSPKFIGHSPQPLGY DRSSRSSYVDEDCDSLALCD PLQRTNCEVDSLPESCFDS
Tissue Specificity Down-regulated in cutaneous melanoma cells but not in breast cancer cells.
Function Binds predominantly GDP, and also GTP. Acts as a dynein adapter protein that activates dynein-mediated transport and dynein-dynactin motility on microtubules.
Protein Name Ras And Ef-Hand Domain-Containing Protein
Ras-Related Protein Rab-45
Cellular Localisation Cytoplasm
Perinuclear Region
Alternative Antibody Names Anti-Ras And Ef-Hand Domain-Containing Protein antibody
Anti-Ras-Related Protein Rab-45 antibody
Anti-RASEF antibody
Anti-RAB45 antibody

Information sourced from Uniprot.org

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