• Immunofluorescence analysis of NIH/3T3 cells using SLC25A27 Rabbit polyclonal antibody (STJ115775) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from mouse brain, using SLC25A27 Rabbit polyclonal antibody (STJ115775) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SLC25A27 antibody (1-245) (STJ115775)

SKU:
STJ115775

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SLC25A27 (1-245) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SLC25A27
Gene ID: 9481
Uniprot ID: UCP4_HUMAN
Immunogen Region: 1-245
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-245 of human SLC25A27 (NP_001190981.1).
Immunogen Sequence: MSVPEEEERLLPLTQRWPRA SKFLLSGCAATVAELATFPL DLTKTRLQMQGEAALARLGD GARESAPYRGMVRTALGIIE EEGFLKLWQGVTPAIYRHVV YSGGRMVTYEHLREVVFGKS EDEHYPLWKSVIGGMMAGVI GQFLANPTDLVKVQMQMEGK RKLEGKPLRFRGVHHAFAKI LAEGGIRGLWAGWVPNIQRA ALVNMGDLTTYDTVKHYLVL NTPLEDNIMTHGLSSDLVG
Tissue Specificity Found in adult and fetal brain. Present in most of the brain tissues, with low levels in spinal chord, corpus callosum and substantia nigra.
Function Facilitates proton transport across the inner mitochondrial membrane and may dissipate excessive proton gradient associated with oxidative and metabolic stress at neuronal synapses. Regulates glutamate-induced proton conductance in astrocytes, shifting the energy metabolism toward aerobic glycolysis and lactate transfer to neurons for ATP synthesis. Can transport chloride ions with lower efficiency. The transport mechanism remains to be elucidated.
Protein Name Mitochondrial Uncoupling Protein 4
Ucp 4
Solute Carrier Family 25 Member 27
Database Links Reactome: R-HSA-167826
Reactome: R-HSA-167827
Cellular Localisation Mitochondrion Inner Membrane
Multi-Pass Membrane Protein
Localizes To Neuronal Cell Body And Processes
Within Mitochondrial Inner Membrane
It Is Mainly Observed In The Inner Boundary Membrane Locally Separated From F(1)F(0) Atp Synthase
Which Is Preferentially Localized In Cristae
Alternative Antibody Names Anti-Mitochondrial Uncoupling Protein 4 antibody
Anti-Ucp 4 antibody
Anti-Solute Carrier Family 25 Member 27 antibody
Anti-SLC25A27 antibody
Anti-UCP4 antibody
Anti-UNQ772 antibody
Anti-PRO1566 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance